ID 4.6.1.14 DE glycosylphosphatidylinositol diacylglycerol-lyase. AN (glycosyl)phosphatidylinositol-specific phospholipase C. AN 6-(alpha-D-glucosaminyl)-1-phosphatidyl-1D-myo-inositol diacylglycerol- AN lyase (1,2-cyclic-phosphate-forming). AN glycosyl inositol phospholipid anchor-hydrolyzing enzyme. AN glycosylphosphatidylinositol-phospholipase C. AN glycosylphosphatidylinositol-specific phospholipase C. AN GPI-PLC. AN GPI-specific phospholipase C. AN variant-surface-glycoprotein phospholipase C. AN VSG-lipase. CA an alpha-D-GlcN-(1->6)-(1,2-diacyl-sn-glycero-3-phospho)-1D-myo-inositol CA = 6-(alpha-D-glucosaminyl)-1D-myo-inositol 1,2-cyclic phosphate + a 1,2- CA diacyl-sn-glycerol. CC -!- This enzyme is also active when O-4 of the glucosamine is substituted CC by carrying the oligosaccharide that can link a protein to the CC structure. CC -!- It therefore cleaves proteins from the lipid part of the CC glycosylphostphatidylinositol (GPI) anchors. CC -!- In some cases, the long-chain acyl group at the sn-1 position of CC glycerol is replaced by an alkyl or alk-1-enyl group. CC -!- In other cases, the diacylglycerol is replaced by ceramide. CC -!- The only characterized enzyme with this specificity is from CC Trypanosoma brucei, where the acyl groups are myristoyl, but the CC function of the trypanosome enzyme is unknown. CC -!- Substitution on O-2 of the inositol blocks action of this enzyme. CC -!- It is not identical with EC 3.1.4.50. CC -!- Formerly EC 3.1.4.47. DR P09194, PHLC_TRYBB ; O15886, PHLC_TRYCR ; //