ID   4.8.1.4
DE   phenylacetaldoxime dehydratase.
AN   (Z)-phenylacetaldehyde-oxime hydro-lyase.
AN   arylacetaldoxime dehydratase.
AN   PAOx dehydratase.
CA   (Z)-phenylacetaldehyde oxime = H2O + phenylacetonitrile.
CC   -!- The enzyme from Bacillus sp. OxB-1 contains protoheme IX, the iron of
CC       which must be in the form iron(II) for activity.
CC   -!- (Z)-Phenylacetaldoxime binds to ferric heme (the iron(III) form) via
CC       the oxygen atom whereas it binds to the active ferrous form via the
CC       nitrogen atom.
CC   -!- In this way, the oxidation state of the heme controls the
CC       coordination stucture of the substrate--heme complex, which regulates
CC       enzyme activity.
CC   -!- The enzyme is active toward several (Z)-arylacetaldoximes and (E/Z)-
CC       alkylaldoximes as well as toward arylalkylaldoximes such as
CC       3-phenylpropionaldoxime and 4-phenylbutyraldoxime.
CC   -!- However, it is inactive with phenylacetaldoximes that have a
CC       substituent group at an alpha-site of an oxime group, for example,
CC       with (E/Z)-2-phenylpropionaldoxime and (E/Z)-mandelaldoxime.
CC   -!- The activity of the enzyme is inhibited completely by the heavy-metal
CC       cations Cu(+), Cu(2+), Ag(+) and Hg(+) whereas Fe(2+) and Sn(2+) have
CC       an activatory effect.
CC   -!- Formerly EC 4.99.1.7.
DR   P82604, OXD_BACSX  ;
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