ID   6.1.1.24
DE   glutamate--tRNA(Gln) ligase.
AN   nondiscriminating glutamyl-tRNA synthetase.
CA   ATP + L-glutamate + tRNA(Glx) = AMP + diphosphate + L-glutamyl-tRNA(Glx).
CC   -!- When this enzyme acts on tRNA(Glu), it catalyzes the same reaction as
CC       EC 6.1.1.17.
CC   -!- It has, however, diminished discrimination, so that it can also form
CC       glutamate-tRNA(Gln).
CC   -!- This relaxation of specificity has been found to result from the
CC       absence of a loop in the tRNA that specifically recognizes the third
CC       position of the anticodon.
CC   -!- This accounts for the ability of this enzyme in, for example,
CC       Bacillus subtilis, to recognize both tRNA1(Gln) (UUG anticodon) and
CC       tRNA(Glu) (UUC anticodon) but not tRNA2(Gln) (CUG anticodon).
CC   -!- The ability of this enzyme to recognize both tRNA(Glu) and one of the
CC       tRNA(Gln) isoacceptors derives from their sharing a major identity
CC       element, a hypermodified derivative of U34 (5-methylaminomethyl-2-
CC       thiouridine).
CC   -!- The glutamyl-tRNA(Gln) is not used in protein synthesis until it is
CC       converted by EC 6.3.5.7 into glutaminyl-tRNA(Gln).
DR   Q5ZJ66, SYEM_CHICK ;  Q0P499, SYEM_DANRE ;  Q5JPH6, SYEM_HUMAN ;
DR   Q4R4F1, SYEM_MACFA ;  Q9CXJ1, SYEM_MOUSE ;  Q66JG3, SYEM_XENTR ;
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