ID 6.1.1.27 DE O-phosphoserine--tRNA ligase. AN O-phosphoseryl-tRNA ligase. AN SepRS. CA ATP + O-phospho-L-serine + tRNA(Cys) = AMP + diphosphate + O-phospho-L- CA seryl-tRNA(Cys). CC -!- In organisms like Archaeoglobus fulgidus lacking EC 6.1.1.16 for the CC direct Cys-tRNA(Cys) formation, Cys-tRNA(Cys) is produced by an CC indirect pathway, in which EC 6.1.1.27 ligates O-phosphoserine to CC tRNA(Cys), and EC 2.5.1.73 converts the produced O-phospho-L-seryl- CC tRNA(Cys) to Cys-tRNA(Cys). CC -!- The SepRS/SepCysS pathway is the sole route for cysteine biosynthesis CC in the organism. CC -!- Methanosarcina mazei can use both pathways, the direct route using CC EC 6.1.1.16 and the indirect pathway with EC 6.1.1.27 and CC EC 2.5.1.73. CC -!- Formerly EC 6.1.1.n2. DR O30126, SEPS_ARCFU ; A6UVW6, SEPS_META3 ; Q8TUH7, SEPS_METAC ; DR Q0W0T4, SEPS_METAR ; A7I4Y4, SEPS_METB6 ; Q46D71, SEPS_METBF ; DR Q12XG0, SEPS_METBU ; Q2FSR2, SEPS_METHJ ; Q59054, SEPS_METJA ; DR Q8TY66, SEPS_METKA ; A2SQV5, SEPS_METLZ ; A4FYB1, SEPS_METM5 ; DR A9A794, SEPS_METM6 ; A6VJZ5, SEPS_METM7 ; Q8PX39, SEPS_METMA ; DR A3CXN8, SEPS_METMJ ; Q6LZE1, SEPS_METMP ; B8GJL9, SEPS_METPE ; DR O27545, SEPS_METTH ; A0B632, SEPS_METTP ; A6USJ0, SEPS_METVS ; //