ID 6.2.1.39 DE [butirosin acyl-carrier protein]--L-glutamate ligase. AN [BtrI acyl-carrier protein]--L-glutamate ligase. CA (1) ATP + holo-[BtrI ACP] + L-glutamate = ADP + gamma-L-glutamyl-[BtrI CA ACP] + phosphate. CA (2) 4-aminobutanoyl-[BtrI ACP] + ATP + L-glutamate = 4-(gamma-L- CA glutamylamino)butanoy-[BtrI ACP] + ADP + H(+) + phosphate. CC -!- Catalyzes two steps in the biosynthesis of the side chain of the CC aminoglycoside antibiotics of the butirosin family. CC -!- The enzyme adds one molecule of L-glutamate to a dedicated acyl- CC carrier protein, and following decarboxylation of the product by CC EC 4.1.1.95, adds a second L-glutamate molecule. DR Q4H4E7, BTRJ_NIACI ; //