ID 6.3.1.9 DE trypanothione synthase. AN TSR synthetase. CA (1) ATP + glutathione + spermidine = ADP + glutathionylspermidine + CA H(+) + phosphate. CA (2) ATP + glutathione + glutathionylspermidine = ADP + H(+) + phosphate + CA trypanothione. CC -!- The enzyme, characterized from several trypanosomatids (e.g. CC Trypanosoma cruzi) catalyzes two subsequent reactions, leading to CC production of trypanothione from glutathione and spermidine. CC -!- Some trypanosomatids (e.g. Crithidia species and some Leishmania CC species) also contain an enzyme that only carries out the first CC reaction (cf. EC 6.3.1.8). CC -!- The enzyme is bifunctional, and also catalyzes the hydrolysis of CC glutathionylspermidine and trypanothione (cf. EC 3.5.1.78). DR O60993, TRYS_CRIFA ; Q9GT49, TRYS_TRYCC ; //