ID 6.3.2.25 DE tubulin--tyrosine ligase. AN TTL. CA ATP + C-terminal L-alpha-aminoacyl-L-glutamyl-L-glutamyl-[tubulin] + CA L-tyrosine = ADP + C-terminal L-alpha-aminoacyl-L-glutamyl-L-glutamyl-L- CA tyrosyl-[tubulin] + H(+) + phosphate. CC -!- L-Tyrosine is linked via a peptide bond to the C-terminus of CC de-tyrosinated alpha-tubulin (des-Tyr(omega)-alpha-tubulin). CC -!- The enzyme is highly specific for alpha-tubulin and moderately CC specific for ATP and L-tyrosine. CC -!- L-Phenylalanine and 3,4-dihydroxy-L-phenylalanine are transferred but CC with higher Km values. DR P38584, TTL_BOVIN ; Q8NG68, TTL_HUMAN ; P38585, TTL_MOUSE ; DR P38160, TTL_PIG ; Q9QXJ0, TTL_RAT ; Q10438, TTL_SCHPO ; DR P38254, TTL_YEAST ; //