ID 6.3.5.4 DE asparagine synthase (glutamine-hydrolyzing). AN AS-B. AN asparagine synthetase (glutamine-hydrolyzing). AN asparagine synthetase B. AN glutamine-dependent asparagine synthetase. CA ATP + H2O + L-aspartate + L-glutamine = AMP + diphosphate + H(+) + CA L-asparagine + L-glutamate. CC -!- The enzyme from Escherichia coli has two active sites that are CC connected by an intramolecular ammonia tunnel. CC -!- The enzyme catalyzes three distinct chemical reactions: glutamine CC hydrolysis to yield ammonia takes place in the N-terminal domain. CC -!- The C-terminal active site mediates both the synthesis of a beta- CC aspartyl-AMP intermediate and its subsequent reaction with ammonia. CC -!- The ammonia released is channeled to the other active site to yield CC asparagine. DR P54420, ASNB_BACSU ; P22106, ASNB_ECOLI ; Q58516, ASNH1_METJA; DR Q58456, ASNH2_METJA; P42113, ASNH_BACSU ; P64248, ASNH_MYCBO ; DR P9WN32, ASNH_MYCTO ; P9WN33, ASNH_MYCTU ; O05272, ASNO_BACSU ; DR P49078, ASNS1_ARATH; P49092, ASNS1_LOTJA; Q10MX3, ASNS1_ORYSJ; DR P19251, ASNS1_PEA ; P49089, ASNS1_YEAST; Q9LV77, ASNS2_ARATH; DR P49093, ASNS2_LOTJA; Q43011, ASNS2_ORYSJ; P19252, ASNS2_PEA ; DR P49090, ASNS2_YEAST; Q9LFU1, ASNS3_ARATH; P31752, ASNS_ASPOF ; DR Q1LZA3, ASNS_BOVIN ; P49091, ASNS_BRAOL ; Q5ZJU3, ASNS_CHICK ; DR P19891, ASNS_CRIGR ; Q54MB4, ASNS_DICDI ; P08243, ASNS_HUMAN ; DR P49094, ASNS_MAIZE ; P17714, ASNS_MESAU ; Q5UQE1, ASNS_MIMIV ; DR Q61024, ASNS_MOUSE ; Q5R6W9, ASNS_PONAB ; P49088, ASNS_RAT ; DR O24338, ASNS_SANAU ; P78753, ASNS_SCHPO ; O24661, ASNS_TRIVS ; //