ID   6.3.5.6
DE   asparaginyl-tRNA synthase (glutamine-hydrolyzing).
AN   Asp-AdT.
AN   aspartyl-tRNA(Asn) amidotransferase.
AN   Asp-tRNA(Asn) amidotransferase.
AN   GatCAB.
CA   ATP + H2O + L-aspartyl-tRNA(Asn) + L-glutamine = ADP + 2 H(+) +
CA   L-asparaginyl-tRNA(Asn) + L-glutamate + phosphate.
CC   -!- This reaction forms part of a two-reaction system for producing
CC       asparaginyl-tRNA in Deinococcus radiodurans and other organisms
CC       lacking a specific enzyme for asparagine synthesis.
CC   -!- In the first step, a non-discriminating ligase (EC 6.1.1.23)
CC       mischarges tRNA(Asn) with aspartate, leading to the formation of
CC       aspartyl-tRNA(Asn).
CC   -!- The aspartyl-tRNA(Asn) is not used in protein synthesis until the
CC       present enzyme converts it into asparaginyl-tRNA(Asn) (aspartyl-
CC       tRNA(Asp) is not a substrate for this enzyme).
CC   -!- A glutaminase subunit (cf. EC 3.5.1.2) produces an ammonia molecule
CC       that is transferred by a 30 A tunnel to a synthase subunit, where it
CC       is ligated to the carboxy group that has been activated by
CC       phosphorylation.
CC   -!- Bacterial GatCAB complexes also has the activity of EC 6.3.5.7.
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