ID   6.5.1.8
DE   3'-phosphate/5'-hydroxy nucleic acid ligase.
CA   (1) a 3'-end 3'-phospho-ribonucleotide-RNA + a 5'-end dephospho-
CA   ribonucleoside-RNA + GTP = a ribonucleotidyl-ribonucleotide-RNA +
CA   diphosphate + GMP.
CA   (2) a 3'-end 2',3'-cyclophospho-ribonucleotide-RNA + a 5'-end dephospho-
CA   ribonucleoside-RNA + GTP + H2O = a ribonucleotidyl-ribonucleotide-RNA +
CA   diphosphate + GMP + H(+).
CC   -!- The enzyme is a 3'-5' nucleic acid ligase with the ability to join
CC       RNA with 3'-phosphate or 2',3'-cyclic-phosphate ends to RNA with
CC       5'-hydroxy ends.
CC   -!- It can also join DNA with 3'-phosphate ends to DNA with 5'-hydroxy
CC       ends, provided the DNA termini are unpaired.
CC   -!- The enzyme is found in members of all three kingdoms of life, and is
CC       essential in metazoa for the splicing of intron-containing tRNAs.
CC   -!- The reaction follows a three-step mechanism with initial activation
CC       of the enzyme by GTP hydrolysis, forming a phosphoramide bond between
CC       the guanylate and a histidine residue.
CC   -!- The guanylate group is transferred to the 3'-phosphate terminus of
CC       the substrate, forming the capped structure [DNA/RNA]-
CC       3'-(5'-diphosphoguanosine).
CC   -!- When a suitable 5'-OH end is available, the enzyme catalyzes an
CC       attack of the 5'-OH on the capped end to form a 3'-5' phosphodiester
CC       splice junction, releasing the guanylate.
CC   -!- When acting on an RNA 2',3'-cyclic-phosphate, the enzyme catalyzes an
CC       additional reaction, hydrolyzing the cyclic phosphate to a
CC       3'-phosphate.
CC   -!- The metazoan enzyme requires activating cofactors in order to achieve
CC       multiple turnover catalysis.
DR   B0WCT9, RTCB1_CULQU;  P0DX92, RTCB1_ECOS1;  B0EAV2, RTCB1_ENTDS;
DR   C4M244, RTCB1_ENTH1;  B0XKF3, RTCB2_CULQU;  P0DX91, RTCB2_ECOS1;
DR   B0EIW5, RTCB2_ENTDS;  C4M6T2, RTCB2_ENTH1;  Q17FP1, RTCB_AEDAE ;
DR   Q9YB37, RTCB_AERPE ;  Q7Q412, RTCB_ANOGA ;  O29399, RTCB_ARCFU ;
DR   Q5E9T9, RTCB_BOVIN ;  C3YN79, RTCB_BRAFL ;  A8QC60, RTCB_BRUMA ;
DR   P90838, RTCB_CAEEL ;  A8JC00, RTCB_CHLRE ;  Q6NZS4, RTCB_DANRE ;
DR   Q54Y09, RTCB_DICDI ;  Q9VIW7, RTCB_DROME ;  P46850, RTCB_ECOLI ;
DR   Q9Y3I0, RTCB_HUMAN ;  Q4R6X4, RTCB_MACFA ;  Q58095, RTCB_METJA ;
DR   Q8TUS2, RTCB_METKA ;  Q6LXF9, RTCB_METMP ;  O27634, RTCB_METTH ;
DR   C1E9Y5, RTCB_MICCC ;  C1MI97, RTCB_MICPC ;  A9UXG6, RTCB_MONBE ;
DR   Q99LF4, RTCB_MOUSE ;  P59975, RTCB_MYCBO ;  P9WGW4, RTCB_MYCTO ;
DR   P9WGW5, RTCB_MYCTU ;  Q74MJ0, RTCB_NANEQ ;  A7RKF6, RTCB_NEMVE ;
DR   A4S3S3, RTCB_OSTLU ;  Q00ZY2, RTCB_OSTTA ;  A9CB42, RTCB_PAPAN ;
DR   Q19PY3, RTCB_PIG   ;  Q4YUZ9, RTCB_PLABA ;  Q8IIU6, RTCB_PLAF7 ;
DR   B3L4K9, RTCB_PLAKH ;  Q7RI54, RTCB_PLAYO ;  Q9V168, RTCB_PYRAB ;
DR   Q8ZY09, RTCB_PYRAE ;  Q8U0H4, RTCB_PYRFU ;  O59245, RTCB_PYRHO ;
DR   Q6AYT3, RTCB_RAT   ;  B8LBM8, RTCB_THAPS ;  Q4U923, RTCB_THEAN ;
DR   Q5JCZ1, RTCB_THEKO ;  Q4N1R8, RTCB_THEPA ;  B3RID0, RTCB_TRIAD ;
DR   Q561P3, RTCB_XENTR ;
//