ENZYME entry: EC 1.14.13.96

Accepted Name
5-beta-cholestane-3-alpha,7-alpha-diol 12-alpha-hydroxylase.
Alternative Name(s)
5-beta-cholestane-3-alpha,7-alpha-diol 12-alpha-monooxygenase.
Cytochrome P450 8B1.
Sterol 12-alpha-hydroxylase.
Reaction catalysed
5-beta-cholestane-3-alpha,7-alpha-diol + NADPH + O(2) <=> 5-beta-cholestane-3-alpha,7-alpha,12-alpha-triol + NADP(+) + H(2)O
Cofactor(s)
Heme-thiolate.
Comment(s)
  • Key enzyme in the biosynthesis of the bile acid cholic acid (3-alpha,7-alpha,12-alpha-trihydroxy-5-beta-cholanoic acid).
  • The activity determines the biosynthetic ratio between cholic acid and chenodeoxycholic acid.
  • Can also hydroxylate the substrate at the 25 and 26 position, but to a lesser extent.
Cross-references
PROSITEPDOC00081
BRENDA1.14.13.96
EC2PDB1.14.13.96
ExplorEnz1.14.13.96
PRIAM enzyme-specific profiles1.14.13.96
KEGG Ligand Database for Enzyme Nomenclature1.14.13.96
IUBMB Enzyme Nomenclature1.14.13.96
IntEnz1.14.13.96
MEDLINEFind literature relating to 1.14.13.96
MetaCyc1.14.13.96
UniProtKB/Swiss-Prot
Q7YRB2, CP8B1_PIG

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