ENZYME

ENZYME entry: EC 1.4.1.13

Accepted Name

Glutamate synthase (NADPH).

Alternative Name(s)

Glutamate (reduced nicotinamide adenine dinucleotide phosphate) synthase.

Glutamate synthetase (NADP).

Glutamine amide-2-oxoglutarate aminotransferase (oxidoreductase, NADP).

Glutamine-ketoglutaric aminotransferase.

GOGAT.

L-glutamate synthase.

L-glutamate synthetase.

L-glutamine:2-oxoglutarate aminotransferase, NADPH oxidizing.

NADPH-dependent glutamate synthase.

NADPH-glutamate synthase.

Reaction catalysed

2 L-glutamate + NADP(+) <=> L-glutamine + 2-oxoglutarate + NADPH

Cofactor(s)

FAD; FMN; Iron-sulfur.

Comment(s)

The reaction takes place in the opposite direction.
The protein is composed of two subunits, alpha and beta.
The alpha subunit is composed of two domains, one hydrolyzing L-glutamine to NH(3) and L-glutamate (cf. EC 3.5.1.2), the other combining the produced NH(3) with 2-oxoglutarate to produce a second molecule of L-glutamate (cf. EC 1.4.1.4).
The beta subunit transfers electrons to the cosubstrate.
The NH(3) is channeled through a 31 A channel in the active protein.
In the absence of the beta subunit, coupling between the two domains of the alpha subunit is compromised and some ammonium can be produced.
In the intact alpha-beta complex, ammonia production only takes place as part of the overall reaction.
Formerly EC 2.6.1.53.

Cross-references

PROSITE

BRENDA

EC2PDB

ExplorEnz

PRIAM enzyme-specific profiles

KEGG Ligand Database for Enzyme Nomenclature

IUBMB Enzyme Nomenclature

IntEnz

MEDLINE

MetaCyc

UniProtKB/Swiss-Prot

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