| Accepted Name |
|---|
| glycine cleavage system
|
| Alternative Name(s) |
|---|
| GCV |
| Reaction catalysed |
|---|
| (6S)-5,6,7,8-tetrahydrofolate + glycine + NAD(+) = (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + CO2 + NADH + NH4(+) |
| Comment(s) |
|---|
- The glycine cleavage (GCV) system is a large multienzyme complex that
belongs to the 2-oxoacid dehydrogenase complex family, which also
includes EC 1.2.1.25, EC 1.2.1.105, EC 1.2.1.104 and EC 2.3.1.190.
- The GCV system catalyzes the reversible oxidation of glycine,
yielding carbon dioxide, ammonia, 5,10-methylenetetrahydrofolate and
a reduced pyridine nucleotide.
- Tetrahydrofolate serves as a recipient for one-carbon units generated
during glycine cleavage to form the methylene group.
- The GCV system consists of four protein components, the P protein
(EC 1.4.4.2), T protein (EC 2.1.2.10), L protein (EC 1.8.1.4),
and the non-enzyme H protein (lipoyl-carrier protein).
- The P protein catalyzes the pyridoxal phosphate-dependent liberation
of CO2 from glycine, leaving a methylamine moiety.
- The methylamine moiety is transferred to the lipoic acid group of the
H protein, which is bound to the P protein prior to decarboxylation
of glycine.
- The T protein catalyzes the release of ammonia from the methylamine
group and transfers the remaining C1 unit to tetrahydrofolate,
forming 5,10-methylenetetrahydrofolate.
- The L protein then oxidizes the lipoic acid component of the H
protein and transfers the electrons to NAD(+), forming NADH.
|
| Cross-references |
|---|
| BRENDA | 1.4.1.27 |
| EC2PDB | 1.4.1.27 |
| ExplorEnz | 1.4.1.27 |
| PRIAM enzyme-specific profiles | 1.4.1.27 |
| KEGG Ligand Database for Enzyme Nomenclature | 1.4.1.27 |
| IUBMB Enzyme Nomenclature | 1.4.1.27 |
| IntEnz | 1.4.1.27 |
| MEDLINE | Find literature relating to 1.4.1.27 |
| MetaCyc | 1.4.1.27 |
| Rhea expert-curated reactions | 1.4.1.27 |
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All ENZYME / UniProtKB/Swiss-Prot entries corresponding to 1.4.-.-
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