ENZYME entry: EC 2.5.1.3

Accepted Name
Thiamine phosphate synthase.
Alternative Name(s)
Thiamine-phosphate diphosphorylase.
Thiamine-phosphate pyrophosphorylase.
Thiamine-phosphate synthase.
TMP diphosphorylase.
TMP pyrophosphorylase.
Reaction catalysed
  • 4-amino-2-methyl-5-diphosphomethylpyrimidine + 2-((2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene)ethyl phosphate <=> diphosphate + thiamine phosphate + CO(2)
  • 4-amino-2-methyl-5-diphosphomethylpyrimidine + 2-(2-carboxy-4-methylthiazol-5-yl)ethyl phosphate <=> diphosphate + thiamine phosphate + CO(2)
  • 4-amino-2-methyl-5-diphosphomethylpyrimidine + 4-methyl-5-(2-phosphono-oxyethyl)thiazole <=> diphosphate + thiamine phosphate
Comment(s)
  • The enzyme catalyzes the penultimate reaction in thiamine de novo biosynthesis, condensing the pyrimidine and thiazole components.
  • The enzyme is thought to accept the product of EC 2.8.1.10 as its substrate.
  • However, it has been shown that in some bacteria, such as Bacillus subtilis, an additional enzyme, EC 5.3.99.10 converts that compound into its tautomer 2-(2-carboxy-4-methylthiazol-5-yl)ethyl phosphate, and that it is the latter that serves as the substrate for the synthase.
  • In addition to this activity, the enzyme participates in a salvage pathway, acting on 4-methyl-5-(2-phosphono-oxyethyl)thiazole, which is produced from thiamine degradation products.
  • In yeast this activity is found in a bifunctional enzyme and in the plant Arabidopsis thaliana the activity is part of a trifunctional enzyme.
Cross-references
BRENDA2.5.1.3
EC2PDB2.5.1.3
ExplorEnz2.5.1.3
PRIAM enzyme-specific profiles2.5.1.3
KEGG Ligand Database for Enzyme Nomenclature2.5.1.3
IUBMB Enzyme Nomenclature2.5.1.3
IntEnz2.5.1.3
MEDLINEFind literature relating to 2.5.1.3
MetaCyc2.5.1.3
UniProtKB/Swiss-Prot
P40386, THI6_SCHPO;  P41835, THI6_YEAST;  Q57688, THIDN_METJA;  
Q9WZP7, THIDN_THEMA;  O66833, THIE1_AQUAE;  Q97RS5, THIE1_STRPN;  
Q8DQK4, THIE1_STRR6;  O67378, THIE2_AQUAE;  P66920, THIE2_STRPN;  
P66921, THIE2_STRR6;  Q8G7X1, THIEC_BIFLO;  Q8FTH8, THIED_COREF;  
Q8NQH1, THIED_CORGL;  P61422, THIED_GEOSL;  A0LTK3, THIE_ACIC1;  
B7JBI2, THIE_ACIF2;  B5ERP0, THIE_ACIF5;  B3GX82, THIE_ACTP7;  
A6VPG9, THIE_ACTSZ;  Q8UAS8, THIE_AGRFC;  Q0VSP5, THIE_ALCBS;  
A6TMN5, THIE_ALKMQ;  A8MK92, THIE_ALKOO;  Q2IM25, THIE_ANADE;  
B7GKQ8, THIE_ANOFW;  O28205, THIE_ARCFU;  Q5P6J5, THIE_AROAE;  
A0JYP1, THIE_ARTS2;  A8IGE6, THIE_AZOC5;  A1KB60, THIE_AZOSB;  
C1DMY6, THIE_AZOVD;  C3PBW1, THIE_BACAA;  C3L627, THIE_BACAC;  
A0R981, THIE_BACAH;  Q81Z95, THIE_BACAN;  B7JN72, THIE_BACC0;  
P61410, THIE_BACC1;  C1EVE6, THIE_BACC3;  B7H7H5, THIE_BACC4;  
B7HT70, THIE_BACC7;  A7GKR4, THIE_BACCN;  B9J2L5, THIE_BACCQ;  
Q81IG8, THIE_BACCR;  Q63GK3, THIE_BACCZ;  Q5LCA7, THIE_BACFN;  
Q64TA1, THIE_BACFR;  Q9KCY8, THIE_BACHD;  Q6HP17, THIE_BACHK;  
Q65DK0, THIE_BACLD;  A7ZA58, THIE_BACMF;  A8FIR6, THIE_BACP2;  
Q5WH87, THIE_BACSK;  P39594, THIE_BACSU;  Q8AA13, THIE_BACTN;  
A6L645, THIE_BACV8;  A9VRN4, THIE_BACWK;  Q2KUS6, THIE_BORA1;  
Q7WF72, THIE_BORBR;  Q7W3U2, THIE_BORPA;  Q7W049, THIE_BORPE;  
B9MN52, THIE_CALBD;  A8M9N4, THIE_CALMQ;  Q8R806, THIE_CALS4;  
A4XG66, THIE_CALS8;  A8FMD4, THIE_CAMJ8;  Q9PNL3, THIE_CAMJE;  
A1W068, THIE_CAMJJ;  Q5HU23, THIE_CAMJR;  B0SUK3, THIE_CAUSK;  
A9WDL8, THIE_CHLAA;  Q5L6R5, THIE_CHLAB;  Q824E9, THIE_CHLCV;  
B3ED41, THIE_CHLL2;  Q3B4B1, THIE_CHLL7;  B3QNM2, THIE_CHLP8;  
A1BGM7, THIE_CHLPD;  A4SEP8, THIE_CHLPM;  Q8KD79, THIE_CHLTE;  
Q7P1R3, THIE_CHRVO;  A8AKT0, THIE_CITK8;  Q97LQ9, THIE_CLOAB;  
A7FPT0, THIE_CLOB1;  C3L061, THIE_CLOB6;  A6M1W6, THIE_CLOB8;  
B2UY65, THIE_CLOBA;  B2TQ13, THIE_CLOBB;  A5HYZ3, THIE_CLOBH;  
C1FSC1, THIE_CLOBJ;  B1IEG6, THIE_CLOBK;  A7GAL0, THIE_CLOBL;  
B1KV12, THIE_CLOBM;  B8I3J4, THIE_CLOCE;  A0Q2A0, THIE_CLONN;  
Q0TQV4, THIE_CLOP1;  Q8XKQ8, THIE_CLOPE;  Q0STA1, THIE_CLOPS;  
Q893R0, THIE_CLOTE;  B5Y6I1, THIE_COPPD;  Q8FP55, THIE_COREF;  
Q4JVZ1, THIE_CORJK;  A7MJ80, THIE_CROS8;  Q478V2, THIE_DECAR;  
Q1IX16, THIE_DEIGD;  Q9RYX9, THIE_DEIRA;  B8FUD4, THIE_DESHD;  
Q24XQ0, THIE_DESHY;  C4XIJ2, THIE_DESMR;  B5YA97, THIE_DICT6;  
B8E358, THIE_DICTD;  A7ZUK8, THIE_ECO24;  Q8X6Y0, THIE_ECO57;  
B5Z089, THIE_ECO5E;  A8A793, THIE_ECOHS;  A1AIG4, THIE_ECOK1;  
Q0TA72, THIE_ECOL5;  Q8FB78, THIE_ECOL6;  B1IUQ4, THIE_ECOLC;  
P30137, THIE_ECOLI;  B6I5K4, THIE_ECOSE;  Q1R5V9, THIE_ECOUT;  
A4W5B6, THIE_ENT38;  Q830K5, THIE_ENTFA;  Q2NB00, THIE_ERYLH;  
A5FJJ1, THIE_FLAJ1;  A6GWR9, THIE_FLAPJ;  Q8RI59, THIE_FUSNN;  
Q5KZU0, THIE_GEOKA;  P61411, THIE_GEOSL;  A4IN28, THIE_GEOTN;  
Q7NNK8, THIE_GLOVI;  Q4QNC6, THIE_HAEI8;  A5UA71, THIE_HAEIE;  
A5UGT0, THIE_HAEIG;  P71350, THIE_HAEIN;  Q0I131, THIE_HAES1;  
A1WYL4, THIE_HALHL;  Q5V0G6, THIE_HALMA;  Q18GX9, THIE_HALWD;  
Q1CT27, THIE_HELPH;  Q9ZL01, THIE_HELPJ;  B2USY5, THIE_HELPS;  
O25514, THIE_HELPY;  A9AZD9, THIE_HERA2;  B4U6B2, THIE_HYDS0;  
Q0BXD3, THIE_HYPNA;  A6SUR1, THIE_JANMA;  A6W6A2, THIE_KINRD;  
A6TGQ0, THIE_KLEP7;  Q1ILG3, THIE_KORVE;  Q03NS1, THIE_LACBA;  
Q03CB2, THIE_LACC3;  B3W783, THIE_LACCB;  B2GBV5, THIE_LACF3;  
Q9CG48, THIE_LACLA;  A2RKJ7, THIE_LACLM;  Q02YS6, THIE_LACLS;  
Q890C0, THIE_LACPL;  A5VKA1, THIE_LACRD;  B2G7Q4, THIE_LACRJ;  
Q38ZM1, THIE_LACSS;  C1DCM3, THIE_LARHH;  Q1MRJ6, THIE_LAWIP;  
B0SE83, THIE_LEPBA;  B0SMR9, THIE_LEPBP;  B1MX63, THIE_LEUCK;  
Q92EW5, THIE_LISIN;  C1KZ34, THIE_LISMC;  Q723Y9, THIE_LISMF;  
B8DET0, THIE_LISMH;  Q8YA44, THIE_LISMO;  A0AFC5, THIE_LISW6;  
B1HX34, THIE_LYSSC;  Q65US7, THIE_MANSM;  A1TYG6, THIE_MARHV;  
Q8TMD6, THIE_METAC;  Q0W1L8, THIE_METAR;  A7IA09, THIE_METB6;  
Q466J5, THIE_METBF;  Q602R3, THIE_METCA;  Q1GXW1, THIE_METFK;  
Q2FM63, THIE_METHJ;  A4FX32, THIE_METM5;  A9AAG9, THIE_METM6;  
A6VG83, THIE_METM7;  Q8PS49, THIE_METMA;  A3CS45, THIE_METMJ;  
P61413, THIE_METMP;  A5ULP4, THIE_METS3;  A6UPE6, THIE_METVS;  
Q2RGI8, THIE_MOOTA;  A0QLT6, THIE_MYCA1;  P66917, THIE_MYCBO;  
A1KFN6, THIE_MYCBP;  A4T2R5, THIE_MYCGI;  B8ZU95, THIE_MYCLB;  
Q9ZBL5, THIE_MYCLE;  B2HPZ9, THIE_MYCMM;  P61412, THIE_MYCPA;  
A0QQK7, THIE_MYCS2;  A3PTW9, THIE_MYCSJ;  A1UAB4, THIE_MYCSK;  
Q1BEL9, THIE_MYCSS;  A5TZE0, THIE_MYCTA;  P9WG74, THIE_MYCTO;  
P9WG75, THIE_MYCTU;  A0PRY2, THIE_MYCUA;  A1T2Z5, THIE_MYCVP;  
Q1D282, THIE_MYXXD;  Q3IP34, THIE_NATPD;  Q5F5C2, THIE_NEIG1;  
Q9JWI2, THIE_NEIMA;  Q9JXF7, THIE_NEIMB;  Q0AFV0, THIE_NITEC;  
Q82UQ7, THIE_NITEU;  Q2Y5Q6, THIE_NITMU;  Q3JDH3, THIE_NITOC;  
Q5YNP9, THIE_NOCFA;  B2J6F7, THIE_NOSP7;  Q8YX72, THIE_NOSS1;  
Q8ESZ3, THIE_OCEIH;  P57930, THIE_PASMU;  Q6DAM1, THIE_PECAS;  
Q03GM4, THIE_PEDPA;  A5D4K4, THIE_PELTS;  Q4FN35, THIE_PELUB;  
Q186F8, THIE_PEPD6;  C0QR82, THIE_PERMH;  Q7N964, THIE_PHOLL;  
Q6KZH9, THIE_PICTO;  A2CB21, THIE_PROM3;  Q7VAV5, THIE_PROMA;  
B4EYU2, THIE_PROMH;  Q7V8I3, THIE_PROMM;  Q7V0I3, THIE_PROMP;  
A2BSJ5, THIE_PROMS;  Q48DP2, THIE_PSE14;  A6V0D3, THIE_PSEA7;  
B7V9E0, THIE_PSEA8;  Q02SE4, THIE_PSEAB;  Q9HX40, THIE_PSEAE;  
Q1I4H6, THIE_PSEE4;  Q4K5I7, THIE_PSEF5;  C3K2K2, THIE_PSEFS;  
A4XYV5, THIE_PSEMY;  A5W9G9, THIE_PSEP1;  Q3K6C1, THIE_PSEPF;  
B0KJV8, THIE_PSEPG;  Q88DP1, THIE_PSEPK;  B1J1Y7, THIE_PSEPW;  
Q87VY6, THIE_PSESM;  Q4ZNA1, THIE_PSEU2;  A4VQX9, THIE_PSEU5;  
A5WDP0, THIE_PSYWF;  Q9UZQ5, THIE_PYRAB;  Q8U192, THIE_PYRFU;  
O58878, THIE_PYRHO;  O34294, THIE_RHIEC;  Q7UQH1, THIE_RHOBA;  
B6INN5, THIE_RHOCS;  Q21VL5, THIE_RHOFT;  A7NRF7, THIE_ROSCS;  
A5UUL3, THIE_ROSS1;  Q21MI9, THIE_SACD2;  A4F727, THIE_SACEN;  
B5F1H6, THIE_SALA4;  A8M4E6, THIE_SALAI;  A9MHD7, THIE_SALAR;  
Q57H62, THIE_SALCH;  B5FQK8, THIE_SALDC;  B5QYE8, THIE_SALEP;  
B5RFJ1, THIE_SALG2;  B4TCT2, THIE_SALHS;  B4T0Z5, THIE_SALNS;  
Q5PKA7, THIE_SALPA;  A9N0K5, THIE_SALPB;  B5BJR2, THIE_SALPK;  
Q2S2A8, THIE_SALRD;  B4TQK3, THIE_SALSV;  Q8Z325, THIE_SALTI;  
A4XBL2, THIE_SALTO;  Q9L9I8, THIE_SALTY;  A8G8F3, THIE_SERP5;  
B2TWI0, THIE_SHIB3;  Q31U04, THIE_SHIBS;  Q32AG6, THIE_SHIDS;  
Q0SY07, THIE_SHIF8;  Q83PB9, THIE_SHIFL;  Q3YUZ1, THIE_SHISS;  
A7X4S8, THIE_STAA1;  A6U3H7, THIE_STAA2;  Q2FF35, THIE_STAA3;  
Q2FWG3, THIE_STAA8;  A5IUN7, THIE_STAA9;  Q2YUL2, THIE_STAAB;  
Q5HEA8, THIE_STAAC;  A6QIT5, THIE_STAAE;  P66918, THIE_STAAM;  
P66919, THIE_STAAN;  Q6GEY4, THIE_STAAR;  Q6G7L9, THIE_STAAS;  
Q8NVH5, THIE_STAAW;  Q9RGS5, THIE_STACT;  Q5HMD0, THIE_STAEQ;  
Q8CNK2, THIE_STAES;  Q4L7X3, THIE_STAHJ;  Q49Z39, THIE_STAS1;  
Q3K1L6, THIE_STRA1;  Q8E5W9, THIE_STRA3;  Q8E092, THIE_STRA5;  
Q82AF9, THIE_STRAW;  Q9S2V2, THIE_STRCO;  A4W0K4, THIE_STRS2;  
A5GMH7, THIE_SYNPW;  Q7U5U1, THIE_SYNPX;  Q0IC17, THIE_SYNS3;  
Q3AL68, THIE_SYNSC;  Q0AZ86, THIE_SYNWW;  P72965, THIE_SYNY3;  
Q9HIQ4, THIE_THEAC;  Q8DHK2, THIE_THEEB;  Q47R33, THIE_THEFY;  
Q72KL8, THIE_THET2;  Q5SKG9, THIE_THET8;  Q97BE8, THIE_THEVO;  
Q3SFU7, THIE_THIDA;  B8GLE3, THIE_THISH;  A7N7S3, THIE_VIBCB;  
Q87JW8, THIE_VIBPA;  Q7M9N9, THIE_WOLSU;  Q8PH45, THIE_XANAC;  
Q4UYA4, THIE_XANC8;  Q8P5R7, THIE_XANCP;  A7IGG9, THIE_XANP2;  
Q9PGC4, THIE_XYLFA;  Q87AX6, THIE_XYLFT;  A7FNH7, THIE_YERP3;  
Q1C1U8, THIE_YERPA;  Q8ZAQ1, THIE_YERPE;  Q1CN71, THIE_YERPN;  
A4TS25, THIE_YERPP;  Q66FP6, THIE_YERPS;  Q67T51, THIME_SYMTH;  
O26949, THIN_METTH;  Q5M731, TPS1L_ARATH;  O48881, TPS1_BRANA;  
Q2QWK9, TPS1_ORYSJ;  

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All ENZYME / UniProtKB/Swiss-Prot entries corresponding to 2.5.1.-
All ENZYME / UniProtKB/Swiss-Prot entries corresponding to 2.5.-.-
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