ENZYME entry: EC 3.4.21.114

Accepted Name
Equine arterivirus serine peptidase.
Alternative Name(s)
3C-like Ser protease.
3C-like serine protease.
3CLSP.
Arterivirus NSP4.
Chymotrypsin-like serine proteinase nsp4.
Equine arteritis virus serine peptidase.
Nonstructural protein 4 serine protease.
Reaction catalysed
Cleavage of (Glu/Gln)-|-(Gly/Ser/Ala) in arterivirus replicase translation products ORF1a and ORF1ab
Comment(s)
  • In the equine arterivirus (EAV), the replicase gene is translated into open reading frame 1a (ORF1a) and ORF1ab polyproteins.
  • This enzyme is the main viral proteinase and processes five cleavage sites in the ORF1a protein and three in the ORF1b-encoded part of the ORF1ab protein to yield nonstructural proteins (nsp5-nsp12).
  • It combines the catalytic system of a chymotrypsin-like serine peptidase (His-Asp-Ser catalytic triad) with the substrate specificity of a 3C-like serine peptidase (Glu or Gln) at the P1 position and a small amino-acid residue (Gly, Ser or Ala) at the P1' position.
  • Cleavage of ORF1ab by this enzyme is essential for viral replication.
  • Belongs to peptidase family S32.
Cross-references
BRENDA3.4.21.114
EC2PDB3.4.21.114
ExplorEnz3.4.21.114
PRIAM enzyme-specific profiles3.4.21.114
KEGG Ligand Database for Enzyme Nomenclature3.4.21.114
IUBMB Enzyme Nomenclature3.4.21.114
IntEnz3.4.21.114
MEDLINEFind literature relating to 3.4.21.114
MetaCyc3.4.21.114

View entry in original ENZYME format
View entry in raw text format (no links)
All ENZYME / UniProtKB/Swiss-Prot entries corresponding to 3.4.21.-
All ENZYME / UniProtKB/Swiss-Prot entries corresponding to 3.4.-.-
All ENZYME / UniProtKB/Swiss-Prot entries corresponding to 3.-.-.-