ENZYME

ENZYME entry: EC 3.5.1.87

Accepted Name

N-carbamoyl-L-amino-acid hydrolase.

Alternative Name(s)

L-carbamoylase.

L-N-carbamoylase.

N-carbamoyl-L-amino acid amidohydrolase.

N-carbamoylase.

N-carbamyl L-amino acid amidohydrolase.

Reaction catalysed

N-carbamoyl-L-2-amino acid (a 2-ureido carboxylate) + H(2)O <=> L-2-amino acid + NH(3) + CO(2)

Cofactor(s)

Cobalt or nickel or manganese.

Comment(s)

This enzyme, along with EC 3.5.1.77, EC 5.1.99.5 and EC 3.5.2.2, forms part of the reaction cascade known as the 'hydantoinase process', which allows the total conversion of D,L-5-monosubstituted hydantoins into optically pure D- or L-amino acids.
The enzyme from Alcaligenes xylosoxidans has broad specificity for carbamoyl-L-amino acids, although it is inactive on the carbamoyl derivatives of glutamate, aspartate, arginine, tyrosine or tryptophan.
The enzyme from Sinorhizobium meliloti requires a divalent cation for activity and can hydrolyze N-carbamoyl-L-tryptophan as well as N-carbamoyl L-amino acids with aliphatic substituents.
The enzyme is inactive on derivatives of D-amino acids.
In addition to N-carbamoyl L-amino acids, the enzyme can also hydrolyze formyl and acetyl derivatives to varying degrees.

Cross-references

BRENDA

EC2PDB

ExplorEnz

PRIAM enzyme-specific profiles

KEGG Ligand Database for Enzyme Nomenclature

IUBMB Enzyme Nomenclature