ENZYME entry: EC 3.5.1.87

Accepted Name
N-carbamoyl-L-amino-acid hydrolase.
Alternative Name(s)
L-carbamoylase.
L-N-carbamoylase.
N-carbamoyl-L-amino acid amidohydrolase.
N-carbamoylase.
N-carbamyl L-amino acid amidohydrolase.
Reaction catalysed
N-carbamoyl-L-2-amino acid (a 2-ureido carboxylate) + H(2)O <=> L-2-amino acid + NH(3) + CO(2)
Cofactor(s)
Mn(2+) or cobalt cation or Ni(2+).
Comment(s)
  • This enzyme, along with EC 3.5.1.77, EC 5.1.99.5 and EC 3.5.2.2, forms part of the reaction cascade known as the 'hydantoinase process', which allows the total conversion of D,L-5-monosubstituted hydantoins into optically pure D- or L-amino acids.
  • The enzyme from Alcaligenes xylosoxidans has broad specificity for carbamoyl-L-amino acids, although it is inactive on the carbamoyl derivatives of glutamate, aspartate, arginine, tyrosine or tryptophan.
  • The enzyme from Sinorhizobium meliloti requires a divalent cation for activity and can hydrolyze N-carbamoyl-L-tryptophan as well as N-carbamoyl L-amino acids with aliphatic substituents.
  • The enzyme is inactive on derivatives of D-amino acids.
  • In addition to N-carbamoyl L-amino acids, the enzyme can also hydrolyze formyl and acetyl derivatives to varying degrees.
Cross-references
BRENDA3.5.1.87
EC2PDB3.5.1.87
ExplorEnz3.5.1.87
PRIAM enzyme-specific profiles3.5.1.87
KEGG Ligand Database for Enzyme Nomenclature3.5.1.87
IUBMB Enzyme Nomenclature3.5.1.87
IntEnz3.5.1.87
MEDLINEFind literature relating to 3.5.1.87
MetaCyc3.5.1.87
UniProtKB/Swiss-Prot
P37113, AMAB1_GEOSE;  Q53389, AMAB2_GEOSE;  

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