ENZYME entry: EC 6.3.5.4
| Accepted Name |
|---|
| Asparagine synthase (glutamine-hydrolyzing).
|
| Alternative Name(s) |
|---|
| AS-B. |
| Asparagine synthetase (glutamine-hydrolyzing). |
| Asparagine synthetase B. |
| Glutamine-dependent asparagine synthetase. |
| Reaction catalysed |
|---|
| ATP + L-aspartate + L-glutamine + H(2)O <=> AMP + diphosphate + L-asparagine + L-glutamate |
| Comment(s) |
|---|
- The enzyme from Escherichia coli has two active sites that are
connected by an intramolecular ammonia tunnel.
- The enzyme catalyzes three distinct chemical reactions: glutamine
hydrolysis to yield ammonia takes place in the N-terminal domain.
- The C-terminal active site mediates both the synthesis of a beta-
aspartyl-AMP intermediate and its subsequent reaction with ammonia.
- The ammonia released is channeled to the other active site to yield
asparagine.
|
| Cross-references |
|---|
| PROSITE | PDOC00406 |
| BRENDA | 6.3.5.4 |
| EC2PDB | 6.3.5.4 |
| ExplorEnz | 6.3.5.4 |
| PRIAM enzyme-specific profiles | 6.3.5.4 |
| KEGG Ligand Database for Enzyme Nomenclature | 6.3.5.4 |
| IUBMB Enzyme Nomenclature | 6.3.5.4 |
| IntEnz | 6.3.5.4 |
| MEDLINE | Find literature relating to 6.3.5.4 |
| MetaCyc | 6.3.5.4 |
| UniProtKB/Swiss-Prot |
| P54420, ASNB_BACSU; | P22106, ASNB_ECOLI; | Q58516, ASNH1_METJA; |
| Q58456, ASNH2_METJA; | P42113, ASNH_BACSU; | P64248, ASNH_MYCBO; |
| P64247, ASNH_MYCTU; | O05272, ASNO_BACSU; | P49078, ASNS1_ARATH; |
| P49092, ASNS1_LOTJA; | Q10MX3, ASNS1_ORYSJ; | P19251, ASNS1_PEA; |
| P49089, ASNS1_YEAST; | Q9LV77, ASNS2_ARATH; | P49093, ASNS2_LOTJA; |
| Q43011, ASNS2_ORYSJ; | P19252, ASNS2_PEA; | P49090, ASNS2_YEAST; |
| Q9LFU1, ASNS3_ARATH; | P31752, ASNS_ASPOF; | Q1LZA3, ASNS_BOVIN; |
| P49091, ASNS_BRAOL; | Q5ZJU3, ASNS_CHICK; | P19891, ASNS_CRIGR; |
| Q54MB4, ASNS_DICDI; | P08243, ASNS_HUMAN; | P49094, ASNS_MAIZE; |
| P17714, ASNS_MESAU; | Q5UQE1, ASNS_MIMIV; | Q61024, ASNS_MOUSE; |
| Q5R6W9, ASNS_PONAB; | P49088, ASNS_RAT; | O24338, ASNS_SANAU; |
| P78753, ASNS_SCHPO; | O24661, ASNS_TRIVS; |
|
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