ENZYME

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A new class EC 7, Translocases, has been added to the EC list. It will be part of ENZYME from release 2018_10. Read more about EC 7 here.

ENZYME entry: EC 1.1.99.31

Accepted Name

(S)-mandelate dehydrogenase.

Alternative Name(s)

L(+)-mandelate dehydrogenase.

MDH.

Reaction catalysed

(S)-2-hydroxy-2-phenylacetate + acceptor <=> 2-oxo-2-phenylacetate + reduced acceptor

Comment(s)

While all enzymes of this family oxidize the (S)-enantiomer of an alpha-hydroxy acid to an alpha-oxo acid, the ultimate oxidant (oxygen, intramolecular heme or some other acceptor) depends on the particular enzyme.
Transfers the electron pair from FMNH(2) to a component of the electron transport chain, most probably ubiquinone.
It is part of a metabolic pathway in Pseudomonads that allows these organisms to utilize mandelic acid, derivatized from the common soil metabolite amygdalin, as the sole source of carbon and energy.
Has a large active-site pocket and preferentially binds substrates with longer sidechains, e.g. 2-hydroxyoctanoate rather than 2-hydroxybutyrate.
It also prefers substrates that, like (S)-mandelate, have beta unsaturation, e.g. (indol-3-yl)glycolate compared with (indol-3- yl)lactate.
Esters of mandelate, such as methyl (S)-mandelate, are also substrates.

Cross-references

PROSITE

BRENDA

EC2PDB

ExplorEnz

PRIAM enzyme-specific profiles

KEGG Ligand Database for Enzyme Nomenclature

IUBMB Enzyme Nomenclature

IntEnz

MEDLINE

MetaCyc

UniProtKB/Swiss-Prot

P32953, CYBL_RHOGR;

P20932, MDLB_PSEPU;

All UniProtKB/Swiss-Prot entries referenced in this entry, with possibility to download in different formats, align etc.

All ENZYME / UniProtKB/Swiss-Prot entries corresponding to 1.1.99.-
All ENZYME / UniProtKB/Swiss-Prot entries corresponding to 1.1.-.-
All ENZYME / UniProtKB/Swiss-Prot entries corresponding to 1.-.-.-