ENZYME

ENZYME entry: EC 1.1.99.37

Accepted Name

Methanol dehydrogenase (nicotinoprotein).

Alternative Name(s)

NDMA-dependent methanol dehydrogenase.

Nicotinoprotein methanol dehydrogenase.

Reaction catalysed

Methanol + acceptor <=> formaldehyde + reduced acceptor

Cofactor(s)

Mg(2+); Zn(2+).

Comment(s)

Nicotinoprotein methanol dehydrogenases have a tightly bound NADP+/NADPH cofactor that does not dissociate during the catalytic process.
Instead, the cofactor is regenerated by a second substrate or electron carrier.
While the in vivo electron acceptor is not known, N,N-dimethyl-4- nitrosoaniline (NDMA) can serve this function in vitro.
The enzyme has been detected in several Gram-positive methylotrophic bacteria, including Amycolatopsis methanolica, Rhodococcus rhodochrous and Rhodococcus erythropolis.
These enzymes are decameric, and possess a 5-fold symmetry.
Some of the enzymes can also dismutate formaldehyde to methanol and formate.

Cross-references

BRENDA

EC2PDB

ExplorEnz

PRIAM enzyme-specific profiles

KEGG Ligand Database for Enzyme Nomenclature

IUBMB Enzyme Nomenclature