Expasy logo

ENZYME

ENZYME entry: EC 1.14.13.243

Accepted Name
toluene 2-monooxygenase
Alternative Name(s)
toluene ortho-monooxygenase
Reaction catalysed
  • H(+) + NADH + O2 + toluene = 2-hydroxytoluene + H2O + NAD(+)
  • 2-hydroxytoluene + H(+) + NADH + O2 = 3-methylcatechol + H2O + NAD(+)
Comment(s)
  • The enzyme, characterized from the bacterium Burkholderia cepacia, belongs to a class of nonheme, oxygen-dependent diiron enzymes.
  • It contains a hydroxylase component with two binuclear iron centers, an NADH-oxidoreductase component containing FAD and a [2Fe-2S] iron- sulfur cluster, and a third component involved in electron transfer between the hydroxylase and the reductase.
  • The enzyme dihydroxylates its substrate in two sequential hydroxylations, initially forming 2-methylphenol, which is hydroxylated to 3-methylcatechol.
Cross-references
BRENDA1.14.13.243
EC2PDB1.14.13.243
ExplorEnz1.14.13.243
PRIAM enzyme-specific profiles1.14.13.243
KEGG Ligand Database for Enzyme Nomenclature1.14.13.243
IUBMB Enzyme Nomenclature1.14.13.243
IntEnz1.14.13.243
MEDLINEFind literature relating to 1.14.13.243
MetaCyc1.14.13.243
Rhea expert-curated reactions1.14.13.243

View entry in original ENZYME format
View entry in raw text format (no links)
All ENZYME / UniProtKB/Swiss-Prot entries corresponding to 1.14.13.-
All ENZYME / UniProtKB/Swiss-Prot entries corresponding to 1.14.-.-
All ENZYME / UniProtKB/Swiss-Prot entries corresponding to 1.-.-.-