ENZYME entry: EC 1.14.13.39
| Accepted Name |
|---|
| Nitric-oxide synthase (NADPH).
|
| Alternative Name(s) |
|---|
| Endothelium-derived relaxation factor-forming enzyme. |
| Endothelium-derived relaxing factor synthase. |
| NADPH-diaphorase. |
| Nitric-oxide synthetase. |
| NO synthase. |
| Reaction catalysed |
|---|
| 2 L-arginine + 3 NADPH + 4 O(2) <=> 2 L-citrulline + 2 nitric oxide + 3 NADP(+) + 4 H(2)O |
| Comment(s) |
|---|
- The enzyme consists of linked oxygenase and reductase domains.
- The eukaryotic enzyme binds FAD, FMN, heme (iron protoporphyrin IX)
and tetrahydrobiopterin, and its two domains are linked via a
regulatory calmodulin-binding domain.
- Upon calcium-induced calmodulin binding, the reductase and oxygenase
domains form a complex, allowing electrons to flow from NADPH via FAD
and FMN to the active center.
- The reductase domain of the enzyme from the bacterium Sorangium
cellulosum utilizes a [2Fe-2S] cluster to transfer the electrons from
NADPH to the active center.
- Cf. EC 1.14.14.47.
|
| Cross-references |
|---|
| BRENDA | 1.14.13.39 |
| EC2PDB | 1.14.13.39 |
| ExplorEnz | 1.14.13.39 |
| PRIAM enzyme-specific profiles | 1.14.13.39 |
| KEGG Ligand Database for Enzyme Nomenclature | 1.14.13.39 |
| IUBMB Enzyme Nomenclature | 1.14.13.39 |
| IntEnz | 1.14.13.39 |
| MEDLINE | Find literature relating to 1.14.13.39 |
| MetaCyc | 1.14.13.39 |
| UniProtKB/Swiss-Prot |
| Q66GP9, NOA1_ARATH; | P29475, NOS1_HUMAN; | Q9Z0J4, NOS1_MOUSE; |
| O19132, NOS1_RABIT; | P29476, NOS1_RAT; | Q29498, NOS1_SHEEP; |
| Q27995, NOS2_BOVIN; | O62699, NOS2_CANLF; | Q28314, NOS2_CAPHI; |
| Q92037, NOS2_CARAU; | O54705, NOS2_CAVPO; | Q90703, NOS2_CHICK; |
| P35228, NOS2_HUMAN; | O46660, NOS2_MACMU; | P29477, NOS2_MOUSE; |
| Q92091, NOS2_ONCMY; | P79290, NOS2_PIG; | O19114, NOS2_RABIT; |
| Q06518, NOS2_RAT; | P29473, NOS3_BOVIN; | Q9TUX8, NOS3_CANLF; |
| P97270, NOS3_CAVPO; | P29474, NOS3_HUMAN; | P70313, NOS3_MOUSE; |
| Q28969, NOS3_PIG; | Q62600, NOS3_RAT; | P79209, NOS3_SHEEP; |
| B1B557, NOSL_BOMMO; | O61608, NOS_ANOST; | Q8T8C0, NOS_BOMMO; |
| Q27571, NOS_DROME; | O61309, NOS_LYMST; | Q6YPG5, NOS_ORYSJ; |
| Q26240, NOS_RHOPR; | Q9I9M2, NOS_SQUAC; |
|
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