Expasy logo

ENZYME

ENZYME entry: EC 1.14.14.39

Accepted Name
isoleucine N-monooxygenase
Alternative Name(s)
CYP79D3
CYP79D4
Reaction catalysed
L-isoleucine + 2 O2 + 2 reduced [NADPH--hemoprotein reductase] = (1E,2S)-2-methylbutanal oxime + CO2 + 2 H(+) + 3 H2O + 2 oxidized [NADPH--hemoprotein reductase]
Comment(s)
  • This enzyme, found in plants, catalyzes two successive N-hydroxylations of L-isoleucine, the committed step in the biosynthesis of the cyanogenic glucoside lotaustralin.
  • The product of the two hydroxylations, N,N-dihydroxy-L-isoleucine, is labile and undergoes dehydration followed by decarboxylation, producing the oxime.
  • It is still not known whether the decarboxylation is spontaneous or catalyzed by the enzyme.
  • The enzyme can also accept L-valine, but with a lower activity. cf. EC 1.14.14.38.
  • Formerly EC 1.14.13.117.
Cross-references
BRENDA1.14.14.39
EC2PDB1.14.14.39
ExplorEnz1.14.14.39
PRIAM enzyme-specific profiles1.14.14.39
KEGG Ligand Database for Enzyme Nomenclature1.14.14.39
IUBMB Enzyme Nomenclature1.14.14.39
IntEnz1.14.14.39
MEDLINEFind literature relating to 1.14.14.39
MetaCyc1.14.14.39
Rhea expert-curated reactions1.14.14.39
UniProtKB/Swiss-Prot
Q9M7B8, C79D1_MANESQ9M7B7, C79D2_MANESQ6J541, C79D3_LOTJA
Q6J540, C79D4_LOTJA

View entry in original ENZYME format
View entry in raw text format (no links)
All UniProtKB/Swiss-Prot entries referenced in this entry, with possibility to download in different formats, align etc.
All ENZYME / UniProtKB/Swiss-Prot entries corresponding to 1.14.14.-
All ENZYME / UniProtKB/Swiss-Prot entries corresponding to 1.14.-.-
All ENZYME / UniProtKB/Swiss-Prot entries corresponding to 1.-.-.-