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A new class EC 7, Translocases, has been added to the EC list. It will be part of ENZYME from release 2018_10. Read more about EC 7 here.

ENZYME entry: EC 1.14.14.39

Accepted Name
Isoleucine N-monooxygenase.
Alternative Name(s)
CYP79D3.
CYP79D4.
Reaction catalysed
L-isoleucine + 2 O(2) + 2 [reduced NADPH--hemoprotein reductase] <=> (1E,2S)-2-methylbutanal oxime + 2 [oxidized NADPH--hemoprotein reductase] + CO(2) + 3 H(2)O
Cofactor(s)
Heme-thiolate.
Comment(s)
  • This enzyme, found in plants, catalyzes two successive N-hydroxylations of L-isoleucine, the committed step in the biosynthesis of the cyanogenic glucoside lotaustralin.
  • The product of the two hydroxylations, N,N-dihydroxy-L-isoleucine, is labile and undergoes dehydration followed by decarboxylation, producing the oxime.
  • It is still not known whether the decarboxylation is spontaneous or catalyzed by the enzyme.
  • The enzyme can also accept L-valine, but with a lower activity.
  • Cf. EC 1.14.14.38, valine N-monooxygenase.
  • Formerly EC 1.4.13.117.
Cross-references
BRENDA1.14.14.39
EC2PDB1.14.14.39
ExplorEnz1.14.14.39
PRIAM enzyme-specific profiles1.14.14.39
KEGG Ligand Database for Enzyme Nomenclature1.14.14.39
IUBMB Enzyme Nomenclature1.14.14.39
IntEnz1.14.14.39
MEDLINEFind literature relating to 1.14.14.39
MetaCyc1.14.14.39
UniProtKB/Swiss-Prot
Q6J541, C79D3_LOTJA;  Q6J540, C79D4_LOTJA;  

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All ENZYME / UniProtKB/Swiss-Prot entries corresponding to 1.14.14.-
All ENZYME / UniProtKB/Swiss-Prot entries corresponding to 1.14.-.-
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