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ENZYME

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ENZYME entry: EC 1.14.15.26

Accepted Name
toluene methyl-monooxygenase
Reaction catalysed
  • 2 H(+) + O2 + 2 reduced [2Fe-2S]-[ferredoxin] + toluene = benzyl alcohol + H2O + 2 oxidized [2Fe-2S]-[ferredoxin]
  • 2 H(+) + O2 + p-xylene + 2 reduced [2Fe-2S]-[ferredoxin] = 4-methylbenzyl alcohol + H2O + 2 oxidized [2Fe-2S]-[ferredoxin]
  • 2 H(+) + m-xylene + O2 + 2 reduced [2Fe-2S]-[ferredoxin] = 3-methylbenzyl alcohol + H2O + 2 oxidized [2Fe-2S]-[ferredoxin]
Comment(s)
  • The enzyme, characterized from several Pseudomonas strains, catalyzes the first step in the degradation of toluenes and xylenes.
  • It has a broad substrate specificity and is also active with substituted compounds, such as chlorotoluenes.
  • The electrons are provided by a reductase (EC 1.18.1.3) that transfers electrons from NADH via FAD and an [2Fe-2S] cluster.
  • The enzyme can also act on its products, producing gem-diols that spontaneously dehydrate to form aldehydes.
Cross-references
BRENDA1.14.15.26
EC2PDB1.14.15.26
ExplorEnz1.14.15.26
PRIAM enzyme-specific profiles1.14.15.26
KEGG Ligand Database for Enzyme Nomenclature1.14.15.26
IUBMB Enzyme Nomenclature1.14.15.26
IntEnz1.14.15.26
MEDLINEFind literature relating to 1.14.15.26
MetaCyc1.14.15.26
Rhea expert-curated reactions1.14.15.26
UniProtKB/Swiss-Prot
P21395, XYLM_PSEPU

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