Home  |  Contact

ENZYME entry: EC 1.14.15.26

Accepted Name
Toluene methyl-monooxygenase.
Reaction catalysed
  • Toluene + O(2) + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H(+) <=> benzyl alcohol + 2 oxidized ferredoxin [iron-sulfur] cluster + H(2)O
  • p-xylene + O(2) + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H(+) <=> 4-methylbenzyl alcohol + 2 oxidized ferredoxin [iron-sulfur] cluster + H(2)O
  • m-xylene + O(2) + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H(+) <=> 3-methylbenzyl alcohol + 2 oxidized ferredoxin [iron-sulfur] cluster + H(2)O
Comment(s)
  • The enzyme, characterized from several Pseudomonas strains, catalyzes the first step in the degradation of toluenes and xylenes.
  • It has a broad substrate specificity and is also active with substituted compounds, such as chlorotoluenes.
  • The electrons are provided by a reductase (EC 1.18.1.3) that transfers electrons from NADH via FAD and an [2Fe-2S] cluster.
  • The enzyme can also act on its products, producing gem-diols that spontaneously dehydrate to form aldehydes.
Cross-references
BRENDA1.14.15.26
EC2PDB1.14.15.26
ExplorEnz1.14.15.26
PRIAM enzyme-specific profiles1.14.15.26
KEGG Ligand Database for Enzyme Nomenclature1.14.15.26
IUBMB Enzyme Nomenclature1.14.15.26
IntEnz1.14.15.26
MEDLINEFind literature relating to 1.14.15.26
MetaCyc1.14.15.26
Rhea expert-curated reactions1.14.15.26

View entry in original ENZYME format
View entry in raw text format (no links)
All ENZYME / UniProtKB/Swiss-Prot entries corresponding to 1.14.15.-
All ENZYME / UniProtKB/Swiss-Prot entries corresponding to 1.14.-.-
All ENZYME / UniProtKB/Swiss-Prot entries corresponding to 1.-.-.-