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ENZYME entry: EC 1.14.15.28

Accepted Name
cholest-4-en-3-one 26-monooxygenase [(25R)-3-oxocholest-4-en-26-oate forming].
Reaction catalysed
cholest-4-en-3-one + 5 H(+) + 3 O2 + 6 reduced [2Fe-2S]-[ferredoxin] <=> (25R)-3-oxocholest-4-en-26-oate + 4 H2O + 6 oxidized [2Fe-2S]-[ferredoxin]
Comment(s)
  • This cytochrome P450 (heme-thiolate) enzyme, found in several bacterial pathogens, is involved in degradation of the host cholesterol.
  • It catalyzes the hydroxylation of the C-26 carbon, followed by oxidation of the alcohol to the carboxylic acid via the aldehyde intermediate, initiating the degradation of the alkyl side-chain of cholesterol.
  • The products are exclusively in the (25R) conformation.
  • The enzyme also accepts cholesterol as a substrate.
  • cf. 1.14.15.29.
  • The enzyme can receive electrons from ferredoxin reductase in vitro, its natural electron donor is not known yet.
  • Formerly EC 1.14.13.221.
Cross-references
BRENDA1.14.15.28
EC2PDB1.14.15.28
ExplorEnz1.14.15.28
PRIAM enzyme-specific profiles1.14.15.28
KEGG Ligand Database for Enzyme Nomenclature1.14.15.28
IUBMB Enzyme Nomenclature1.14.15.28
IntEnz1.14.15.28
MEDLINEFind literature relating to 1.14.15.28
MetaCyc1.14.15.28
Rhea expert-curated reactions1.14.15.28
UniProtKB/Swiss-Prot
P0A517, CP124_MYCBO;  P9WPP2, CP124_MYCTO;  P9WPP3, CP124_MYCTU;  
A0R4Q6, CP142_MYCS2;  P9WPL4, CP142_MYCTO;  P9WPL5, CP142_MYCTU;  

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