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ENZYME entry: EC 1.14.15.28
Accepted Name |
cholest-4-en-3-one 26-monooxygenase [(25R)-3-oxocholest-4-en-26-oate
forming].
|
Reaction catalysed |
cholest-4-en-3-one + 5 H(+) + 3 O2 + 6 reduced [2Fe-2S]-[ferredoxin] <=> (25R)-3-oxocholest-4-en-26-oate + 4 H2O + 6 oxidized [2Fe-2S]-[ferredoxin] |
Comment(s) |
- This cytochrome P450 (heme-thiolate) enzyme, found in several
bacterial pathogens, is involved in degradation of the host
cholesterol.
- It catalyzes the hydroxylation of the C-26 carbon, followed by
oxidation of the alcohol to the carboxylic acid via the aldehyde
intermediate, initiating the degradation of the alkyl side-chain of
cholesterol.
- The products are exclusively in the (25R) conformation.
- The enzyme also accepts cholesterol as a substrate.
- cf. 1.14.15.29.
- The enzyme can receive electrons from ferredoxin reductase in vitro,
its natural electron donor is not known yet.
- Formerly EC 1.14.13.221.
|
Cross-references |
BRENDA | 1.14.15.28 |
EC2PDB | 1.14.15.28 |
ExplorEnz | 1.14.15.28 |
PRIAM enzyme-specific profiles | 1.14.15.28 |
KEGG Ligand Database for Enzyme Nomenclature | 1.14.15.28 |
IUBMB Enzyme Nomenclature | 1.14.15.28 |
IntEnz | 1.14.15.28 |
MEDLINE | Find literature relating to 1.14.15.28 |
MetaCyc | 1.14.15.28 |
Rhea expert-curated reactions | 1.14.15.28 |
UniProtKB/Swiss-Prot |
|
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