ENZYME entry: EC 1.14.15.33

Accepted Name

pikromycin synthase

Alternative Name(s)

CYP107L1

Reaction catalysed

narbomycin + 2 reduced [2Fe-2S]-[ferredoxin] + O2 + 2 H(+) = pikromycin + 2 oxidized [2Fe-2S]-[ferredoxin] + H2O
narbomycin + 2 reduced [2Fe-2S]-[ferredoxin] + O2 + 2 H(+) = neopikromycin + 2 oxidized [2Fe-2S]-[ferredoxin] + H2O
narbomycin + 4 reduced [2Fe-2S]-[ferredoxin] + 2 O2 + 4 H(+) = novapikromycin + 4 oxidized [2Fe-2S]-[ferredoxin] + 2 H2O
10-deoxymethymycin + 2 reduced [2Fe-2S]-[ferredoxin] + O2 + 2 H(+) = methymycin + 2 oxidized [2Fe-2S]-[ferredoxin] + H2O
10-deoxymethymycin + 2 reduced [2Fe-2S]-[ferredoxin] + O2 + 2 H(+) = neomethymycin + 2 oxidized [2Fe-2S]-[ferredoxin] + H2O
10-deoxymethymycin + 4 reduced [2Fe-2S]-[ferredoxin] + 2 O2 + 4 H(+) = novamethymycin + 4 oxidized [2Fe-2S]-[ferredoxin] + 2 H2O

Comment(s)

A cytochrome P450 (heme-thiolate) protein.
Involved in the biosynthesis of a number of bacterial macrolide antibiotics containing a desosamine glycoside unit.
With narbomycin it hydroxylates at either C-12 to give pikromycin or C-14 to give neopikromycin or both positions to give narvopikromycin.
With 10-deoxymethymycin it hydroxylates at either C-10 to give methymycin or C-12 to give neomethymycin or both positions to give novamethymycin.
Formerly EC 1.14.13.185.

Cross-references

BRENDA

EC2PDB

ExplorEnz

KEGG Ligand Database for Enzyme Nomenclature

IUBMB Enzyme Nomenclature

MEDLINE

MetaCyc

Rhea expert-curated reactions

UniProtKB/Swiss-Prot

O87605, PIKC_STRVZ

All UniProtKB/Swiss-Prot entries referenced in this entry, with possibility to download in different formats, align etc.

All ENZYME / UniProtKB/Swiss-Prot entries corresponding to 1.14.15.-
All ENZYME / UniProtKB/Swiss-Prot entries corresponding to 1.14.-.-
All ENZYME / UniProtKB/Swiss-Prot entries corresponding to 1.-.-.-