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ENZYME

ENZYME entry: EC 1.16.1.6

PURL: https://purl.expasy.org/enzyme/EC/1.16.1.6
Accepted Name
cyanocobalamin reductase
Alternative Name(s)
cyanocobalamin reductase (cyanide-eliminating)
cyanocobalamin reductase (NADPH, cyanide-eliminating)
cyanocobalamin reductase (NADPH; CN-eliminating)
NADPH:cyanocob(III)alamin oxidoreductase (cyanide-eliminating)
Reaction catalysed
2 cob(II)alamin-[cyanocobalamin reductase] + 2 hydrogen cyanide + NADP(+) = 2 cyanocob(III)alamin + 2 apo-[cyanocobalamin reductase] + NADPH + H(+)
Comment(s)
  • The mammalian enzyme, which is cytosolic, can bind internalized cyanocobalamin and process it to cob(II)alamin by removing the upper axial ligand.
  • The product remains bound to the protein, which, together with its interacting partner MMADHC, transfers it directly to downstream enzymes involved in adenosylcobalamin and methylcobalamin biosynthesis.
  • In addition to its decyanase function, the mammalian enzyme also catalyzes an entirely different chemical reaction with alkylcobalamins, using the thiolate of glutathione for nucleophilic displacement, generating cob(I)alamin and the corresponding glutathione thioether (cf. EC 2.5.1.151).
  • Formerly EC 1.6.99.12.
Cross-references
BRENDA1.16.1.6
EC2PDB1.16.1.6
ExplorEnz1.16.1.6
KEGG Ligand Database for Enzyme Nomenclature1.16.1.6
IUBMB Enzyme Nomenclature1.16.1.6
MEDLINEFind literature relating to 1.16.1.6
MetaCyc1.16.1.6
Rhea expert-curated reactions1.16.1.6
UniProtKB/Swiss-Prot
Q5E9C8, MMAC_BOVINQ5ZL21, MMAC_CHICKQ5RFU5, MMAC_DANRE
Q9Y4U1, MMAC_HUMANQ9CZD0, MMAC_MOUSE

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