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ENZYME entry: EC 1.17.99.3

Accepted Name
3alpha,7alpha,12alpha-trihydroxy-5beta-cholestanoyl-CoA 24-hydroxylase.
Alternative Name(s)
3alpha,7alpha,12alpha-trihydroxy-5beta-cholestan-26-oate 24-hydroxylase.
3alpha,7alpha,12alpha-trihydroxy-5beta-cholestanoyl-CoA oxidase.
THCA-CoA oxidase.
THC-CoA oxidase.
trihydroxycoprostanoyl-CoA oxidase.
Reaction catalysed
(25R)-3alpha,7alpha,12alpha-trihydroxy-5beta-cholestan-26-oyl-CoA + A + H2O <=> (24R,25R)-3alpha,7alpha,12alpha,24-tetrahydroxy-5beta-cholestan-26-oyl-CoA + AH2
Comment(s)
  • Requires ATP.
  • The reaction in mammals possibly involves dehydrogenation to give a 24(25)-double bond followed by hydration.
  • However, in amphibians such as the Oriental fire-bellied toad (Bombina orientalis), it is probable that the product is formed via direct hydroxylation of the saturated side chain of (25R)- 3alpha,7alpha,12alpha-trihydroxy-5beta-cholestan-26-oate and not via hydration of a 24(25) double bond.
  • In microsomes, the free acid is preferred to the coenzyme A ester, whereas in mitochondria, the coenzyme A ester is preferred to the free-acid form of the substrate.
Cross-references
BRENDA1.17.99.3
EC2PDB1.17.99.3
ExplorEnz1.17.99.3
PRIAM enzyme-specific profiles1.17.99.3
KEGG Ligand Database for Enzyme Nomenclature1.17.99.3
IUBMB Enzyme Nomenclature1.17.99.3
IntEnz1.17.99.3
MEDLINEFind literature relating to 1.17.99.3
MetaCyc1.17.99.3
Rhea expert-curated reactions1.17.99.3
UniProtKB/Swiss-Prot
Q99424, ACOX2_HUMAN;  Q9QXD1, ACOX2_MOUSE;  O02767, ACOX2_RABIT;  
P97562, ACOX2_RAT;  

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