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ENZYME

ENZYME entry: EC 2.3.1.290

PURL: https://purl.expasy.org/enzyme/EC/2.3.1.290
Accepted Name
spectinabilin polyketide synthase system
Alternative Name(s)
spectinabilin polyketide synthase complex
Reaction catalysed
4-nitrobenzoyl-CoA + 6 (S)-methylmalonyl-CoA + malonyl-CoA + 6 NADPH + 12 H(+) = demethyldeoxyspectinabilin + 7 CO2 + 6 NADP(+) + 8 CoA + 5 H2O
Comment(s)
  • This polyketide synthase, characterized from the bacteria Streptomyces orinoci and Streptomyces spectabilis, generates the backbone of the antibiotic spectinabilin.
  • It is composed of 6 modules that total 28 domains and is encoded by four genes.
  • The enzyme accepts the unusual starter unit 4-nitrobenzoyl-CoA and extends it by 6 molecules of (S)-methylmalonyl-CoA and a single molecule of malonyl-CoA.
  • The first module (encoded by norA) is used twice in an iterative fashion, so that the seven Claisen condensation reactions are catalyzed by only six modules.
  • The iteration becomes possible by the transfer of the [acp]-bound polyketide intermediate back to the ketosynthase (KS) domain on the opposite polyketide synthase strand (polyketides are homodimeric).
Cross-references
BRENDA2.3.1.290
EC2PDB2.3.1.290
ExplorEnz2.3.1.290
KEGG Ligand Database for Enzyme Nomenclature2.3.1.290
IUBMB Enzyme Nomenclature2.3.1.290
MEDLINEFind literature relating to 2.3.1.290
MetaCyc2.3.1.290
Rhea expert-curated reactions2.3.1.290
UniProtKB/Swiss-Prot
B4ER95, NORA2_STRONB4ER92, NORA_STRONB4ER96, NORB_STRON
B4ER97, NORC_STRON

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