Accepted Name |
(phenol)carboxyphthiodiolenone synthase
|
Reaction catalysed |
- 2 (S)-methylmalonyl-CoA + 10 H(+) + icosanoyl-[(phenol)carboxyphthiodiolenone synthase] + 3 malonyl-CoA + 5 NADPH <=> C32-carboxyphthiodiolenone-[(phenol)carboxyphthiodiolenone synthase] + 5 CO2 + 5 CoA + 2 H2O + 5 NADP(+)
- 2 (S)-methylmalonyl-CoA + docosanoyl-[(phenol)carboxyphthiodiolenone synthase] + 10 H(+) + 3 malonyl-CoA + 5 NADPH <=> C34-carboxyphthiodiolenone-[(phenol)carboxyphthiodiolenone synthase] + 5 CO2 + 5 CoA + 2 H2O + 5 NADP(+)
- 2 (S)-methylmalonyl-CoA + 19-(4-hydroxyphenyl)nonadecanoyl-[(phenol)carboxyphthiodiolenone synthase] + 10 H(+) + 3 malonyl-CoA + 5 NADPH <=> C37-(phenol)carboxyphthiodiolenone-[(phenol)carboxyphthiodiolenone synthase] + 5 CO2 + 5 CoA + 2 H2O + 5 NADP(+)
- 2 (S)-methylmalonyl-CoA + 17-(4-hydroxyphenyl)heptadecanoyl-[(phenol)carboxyphthiodiolenone synthase] + 10 H(+) + 3 malonyl-CoA + 5 NADPH <=> C35-(phenol)carboxyphthiodiolenone-[(phenol)carboxyphthiodiolenone synthase] + 5 CO2 + 5 CoA + 2 H2O + 5 NADP(+)
|
Comment(s) |
- The enzyme, which is a complex of five polyketide synthase proteins,
is involved in the synthesis of the lipid core common to phthiocerols
and phenolphthiocerols.
- The first protein, PpsA, can accept either a C18 or C20 long-chain
fatty acyl, or a (4-hydroxyphenyl)-C17 or C19 fatty acyl.
- The substrates must first be adenylated by EC 6.2.1.59, which also
loads them onto PpsA.
- PpsA then extends them using a malonyl-CoA extender unit.
- The PpsB protein adds the next malonyl-CoA extender unit.
- The absence of a dehydratase and an enoyl reductase domains in the
PpsA and PpsB modules results in the formation of the diol portion of
the phthiocerol moiety.
- PpsC adds a third malonyl unit (releasing a water molecule due to its
dehydratase domain), PpsD adds an (R)-methylmalonyl unit, releasing a
water molecule, and PpsE adds a second (R)-methylmalonyl unit,
without releasing a water molecule.
- The incorporation of the methylmalonyl units results in formation of
two branched methyl groups in the elongated product.
- The enzyme does not contain a thioesterase domain, and release of the
products requires the tesA-encoded type II thioesterase.
|
Cross-references |
BRENDA | 2.3.1.292 |
EC2PDB | 2.3.1.292 |
ExplorEnz | 2.3.1.292 |
PRIAM enzyme-specific profiles | 2.3.1.292 |
KEGG Ligand Database for Enzyme Nomenclature | 2.3.1.292 |
IUBMB Enzyme Nomenclature | 2.3.1.292 |
IntEnz | 2.3.1.292 |
MEDLINE | Find literature relating to 2.3.1.292 |
MetaCyc | 2.3.1.292 |
Rhea expert-curated reactions | 2.3.1.292 |
UniProtKB/Swiss-Prot |
Q7TXM0, PPSA_MYCBO | P9WQE6, PPSA_MYCTO | P9WQE7, PPSA_MYCTU |
Q7TXL9, PPSB_MYCBO | P9WQE4, PPSB_MYCTO | P9WQE5, PPSB_MYCTU |
Q7TXL8, PPSC_MYCBO | P96202, PPSC_MYCTU | Q7TXL7, PPSD_MYCBO |
P9WQE2, PPSD_MYCTO | P9WQE3, PPSD_MYCTU | Q7TXL6, PPSE_MYCBO |
P9WQE0, PPSE_MYCTO | P9WQE1, PPSE_MYCTU |
|
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