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ENZYME entry: EC

Accepted Name
(E3-independent) E2 ubiquitin-conjugating enzyme.
Alternative Name(s)
E3-independent ubiquitin-conjugating enzyme E2.
Reaction catalysed
S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [acceptor protein]-L-lysine <=> [E1 ubiquitin-activating enzyme]-L-cysteine + N(6)-monoubiquitinyl-[acceptor protein]-L-lysine
  • The enzyme transfers a single ubiquitin directly from an ubiquitinated E1 ubiquitin-activating enzyme to itself, and on to a lysine residue of the acceptor protein without involvement of E3 ubiquitin transferases (cf. EC, EC
  • It forms a labile ubiquitin adduct in the presence of E1, ubiquitin, and Mg(2+)-ATP and catalyzes the conjugation of ubiquitin to protein substrates, independently of E3.
  • This transfer has only been observed with small proteins.
  • In vitro a transfer to small acceptors (e.g. L-lysine, N-acetyl-L- lysine methyl ester) has been observed.
PRIAM enzyme-specific profiles2.3.2.24
KEGG Ligand Database for Enzyme Nomenclature2.3.2.24
IUBMB Enzyme Nomenclature2.3.2.24
MEDLINEFind literature relating to
Rhea expert-curated reactions2.3.2.24
Q2TA10, UB2D1_BOVIN;  P51668, UB2D1_HUMAN;  P61080, UB2D1_MOUSE;  
D3ZDK2, UB2D1_RAT;  Q1RMX2, UB2D2_BOVIN;  P62837, UB2D2_HUMAN;  
P62838, UB2D2_MOUSE;  Q06AA9, UB2D2_PIG;  P62839, UB2D2_RAT;  
P62840, UB2D2_XENLA;  Q3ZCF7, UB2D3_BOVIN;  P61077, UB2D3_HUMAN;  
Q4R5N4, UB2D3_MACFA;  P61079, UB2D3_MOUSE;  Q5R4V7, UB2D3_PONAB;  
P61078, UB2D3_RAT;  P51965, UB2E1_HUMAN;  P52482, UB2E1_MOUSE;  
P49427, UB2R1_HUMAN;  Q8CFI2, UB2R1_MOUSE;  Q32PA5, UBE2C_BOVIN;  
Q9D1C1, UBE2C_MOUSE;  Q95044, UBE2C_SPISO;  P56616, UBE2C_XENLA;  
Q32LN1, UBE2H_BOVIN;  P62256, UBE2H_HUMAN;  P62257, UBE2H_MOUSE;  

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