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ENZYME entry: EC 2.3.2.32

Accepted Name
Cullin-RING-type E3 NEDD8 transferase.
Reaction catalysed
S-[NEDD8-protein]-yl-[E2 NEDD8-conjugating enzyme]-L-cysteine + [cullin]-L-lysine <=> [E2 NEDD8-conjugating enzyme]-L-cysteine + N(6)-[NEDD8-protein]-yl-[cullin]-L-lysine
Comment(s)
  • Some RING-type E3 ubiquitin transferase (EC 2.3.2.27) are not able to bind a substrate protein directly.
  • Instead, they form a complex with a cullin scaffold protein and a substrate recognition module, which is named CRL for Cullin-RING- Ligase.
  • The cullin protein needs to be activated by the ubiquitin-like protein NEDD8 in a process known as neddylation.
  • The transfer of NEDD8 from a NEDD8-specific E2 enzyme onto the cullin protein is a secondary function of the RING-type E3 ubiquitin transferase in the CRL complex.
  • The process requires auxiliary factors that belong to the DCN1 (defective in cullin neddylation 1) family.
Cross-references
BRENDA2.3.2.32
EC2PDB2.3.2.32
ExplorEnz2.3.2.32
PRIAM enzyme-specific profiles2.3.2.32
KEGG Ligand Database for Enzyme Nomenclature2.3.2.32
IUBMB Enzyme Nomenclature2.3.2.32
IntEnz2.3.2.32
MEDLINEFind literature relating to 2.3.2.32
MetaCyc2.3.2.32
UniProtKB/Swiss-Prot
P62877, RBX1_HUMAN;  P62878, RBX1_MOUSE;  Q1RMW1, UBE2F_BOVIN;  
Q5ZKX6, UBE2F_CHICK;  Q6NY82, UBE2F_DANRE;  Q969M7, UBE2F_HUMAN;  
Q5U203, UBE2F_RAT;  Q6IRC7, UBE2F_XENLA;  Q5M8Y2, UBE2F_XENTR;  

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