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ENZYME entry: EC

Accepted Name
sterol 27-beta-glucosyltransferase
Reaction catalysed
a 27-hydroxysterol + UDP-alpha-D-glucose <=> a sterol 27-beta-D-glucoside + H(+) + UDP
  • The enzyme, isolated from the plant Withania somnifera (ashwagandha), transfers D-glucose to a beta-hydroxyl group present at the C-27 position in sterols/withanolides, provided the substrate possesses a 17alpha-OH group.
  • Natural substrates are 17alpha-hydroxywithaferin A, 27beta- hydroxywithanone, and 5alpha,6beta,17alpha,27beta- tetrahydroxywithanolide.
  • The enzyme's activity with withanolide A and withanolide U, which lack a 17alpha-hydroxyl group, suggests it may also be able to glucosylate the C-20 beta-OH position, although this has not been verified yet.
  • The enzyme does not glucosylate sterols at the C-3 position.
PRIAM enzyme-specific profiles2.4.1.385
KEGG Ligand Database for Enzyme Nomenclature2.4.1.385
IUBMB Enzyme Nomenclature2.4.1.385
MEDLINEFind literature relating to
Rhea expert-curated reactions2.4.1.385

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