Expasy logo

ENZYME

ENZYME entry: EC 2.4.1.385

PURL: https://purl.expasy.org/enzyme/EC/2.4.1.385
Accepted Name
sterol 27-beta-glucosyltransferase
Reaction catalysed
a 27-hydroxysterol + UDP-alpha-D-glucose = a sterol 27-beta-D-glucoside + UDP + H(+)
Comment(s)
  • The enzyme, isolated from the plant Withania somnifera (ashwagandha), transfers D-glucose to a beta-hydroxyl group present at the C-27 position in sterols/withanolides, provided the substrate possesses a 17alpha-OH group.
  • Natural substrates are 17alpha-hydroxywithaferin A, 27beta- hydroxywithanone, and 5alpha,6beta,17alpha,27beta- tetrahydroxywithanolide.
  • The enzyme's activity with withanolide A and withanolide U, which lack a 17alpha-hydroxyl group, suggests it may also be able to glucosylate the C-20 beta-OH position, although this has not been verified yet.
  • The enzyme does not glucosylate sterols at the C-3 position.
Cross-references
Rhea expert-curated reactions2.4.1.385
ExplorEnz2.4.1.385
IUBMB Enzyme Nomenclature2.4.1.385
BRENDA2.4.1.385
MetaCyc2.4.1.385
KEGG Ligand Database for Enzyme Nomenclature2.4.1.385
MEDLINEFind literature relating to 2.4.1.385

View entry in original ENZYME format
View entry in raw text format (no links)
All ENZYME / UniProtKB/Swiss-Prot entries corresponding to 2.4.1.-
All ENZYME / UniProtKB/Swiss-Prot entries corresponding to 2.4.-.-
All ENZYME / UniProtKB/Swiss-Prot entries corresponding to 2.-.-.-