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ENZYME

ENZYME entry: EC 2.4.99.18

PURL: https://purl.expasy.org/enzyme/EC/2.4.99.18
Accepted Name
dolichyl-diphosphooligosaccharide--protein glycotransferase
Reaction catalysed
a di-trans,poly-cis-dolichyl diphosphooligosaccharide + L-asparaginyl-[protein] = N(4)-(oligosaccharide-(1->4)-N-acetyl-beta-D-glucosaminyl-(1->4)-N-acetyl-beta-D-glucosaminyl)-L-asparaginyl-[protein] + a di-trans,poly-cis-dolichyl diphosphate + H(+)
Comment(s)
  • Occurs in eukaryotes that form a glycoprotein by the transfer of a glucosyl-mannosyl-glucosamine polysaccharide to the side-chain of an L-asparagine residue in the sequence -Asn-Xaa-Ser- or -Asn-Xaa-Thr- (Xaa not Pro) in nascent polypeptide chains.
  • The basic oligosaccharide is the tetradecasaccharide Glc3Man9GlcNAc2.
  • However, smaller oligosaccharides derived from it and oligosaccharides with additional monosaccharide units attached may be involved.
  • Man3GlcNAc2 seems to be common for all of the oligosaccharides involved with the terminal N-acetylglucosamine linked to the protein L-asparagine.
  • Occurs on the lumenal face of the endoplasmic reticulum.
  • The dolichol involved normally has 14-21 isoprenoid units with two trans double-bonds at the omega end, and the rest of the double-bonds in cis form.
  • Formerly EC 2.4.1.119.
Cross-references
BRENDA2.4.99.18
EC2PDB2.4.99.18
ExplorEnz2.4.99.18
KEGG Ligand Database for Enzyme Nomenclature2.4.99.18
IUBMB Enzyme Nomenclature2.4.99.18
MEDLINEFind literature relating to 2.4.99.18
MetaCyc2.4.99.18
Rhea expert-curated reactions2.4.99.18
UniProtKB/Swiss-Prot
Q93ZY3, STT3A_ARATHQ2KJI2, STT3A_BOVINF1PJP5, STT3A_CANLF
P46977, STT3A_HUMANP46978, STT3A_MOUSEQ6F2Z1, STT3A_ORYSJ
Q5RCE2, STT3A_PONABQ9FX21, STT3B_ARATHE2RG47, STT3B_CANLF
Q8TCJ2, STT3B_HUMANQ3TDQ1, STT3B_MOUSEQ7XQ88, STT3B_ORYSJ
P46975, STT3_CAEELQ54NM9, STT3_DICDIO94335, STT3_SCHPO
P39007, STT3_YEAST

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