ENZYME entry: EC 2.4.99.18
Accepted Name |
dolichyl-diphosphooligosaccharide--protein glycotransferase.
|
Reaction catalysed |
a dolichyl diphosphooligosaccharide + L-asparaginyl-[protein] <=> a dolichyl diphosphate + H(+) + N(4)-(oligosaccharide-(1->4)-N-acetyl-beta-D-glucosaminyl-(1->4)-N-acetyl-beta-D-glucosaminyl)-L-asparaginy-[protein] |
Comment(s) |
- Occurs in eukaryotes that form a glycoprotein by the transfer of a
glucosyl-mannosyl-glucosamine polysaccharide to the side-chain of an
L-asparagine residue in the sequence -Asn-Xaa-Ser- or -Asn-Xaa-Thr-
(Xaa not Pro) in nascent polypeptide chains.
- The basic oligosaccharide is the tetradecasaccharide Glc3Man9GlcNAc2.
- However, smaller oligosaccharides derived from it and
oligosaccharides with additional monosaccharide units attached may be
involved.
- Man3GlcNAc2 seems to be common for all of the oligosaccharides
involved with the terminal N-acetylglucosamine linked to the protein
L-asparagine.
- Occurs on the cytosolic face of the endoplasmic reticulum.
- The dolichol involved normally has 14-21 isoprenoid units with two
trans double-bonds at the omega end, and the rest of the double-bonds
in cis form.
- Formerly EC 2.4.1.119.
|
Cross-references |
BRENDA | 2.4.99.18 |
EC2PDB | 2.4.99.18 |
ExplorEnz | 2.4.99.18 |
PRIAM enzyme-specific profiles | 2.4.99.18 |
KEGG Ligand Database for Enzyme Nomenclature | 2.4.99.18 |
IUBMB Enzyme Nomenclature | 2.4.99.18 |
IntEnz | 2.4.99.18 |
MEDLINE | Find literature relating to 2.4.99.18 |
MetaCyc | 2.4.99.18 |
Rhea expert-curated reactions | 2.4.99.18 |
UniProtKB/Swiss-Prot |
Q93ZY3, STT3A_ARATH; | Q2KJI2, STT3A_BOVIN; | F1PJP5, STT3A_CANLF; |
P46977, STT3A_HUMAN; | P46978, STT3A_MOUSE; | Q6F2Z1, STT3A_ORYSJ; |
Q5RCE2, STT3A_PONAB; | Q9FX21, STT3B_ARATH; | E2RG47, STT3B_CANLF; |
Q8TCJ2, STT3B_HUMAN; | Q3TDQ1, STT3B_MOUSE; | Q7XQ88, STT3B_ORYSJ; |
P46975, STT3_CAEEL; | Q54NM9, STT3_DICDI; | O94335, STT3_SCHPO; |
P39007, STT3_YEAST; |
|
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