ENZYME entry: EC 2.4.99.18
| Accepted Name |
|---|
| dolichyl-diphosphooligosaccharide--protein glycotransferase.
|
| Reaction catalysed |
|---|
| a dolichyl diphosphooligosaccharide + L-asparaginyl-[protein] <=> a dolichyl diphosphate + H(+) + N(4)-(oligosaccharide-(1->4)-N-acetyl-beta-D-glucosaminyl-(1->4)-N-acetyl-beta-D-glucosaminyl)-L-asparaginy-[protein] |
| Comment(s) |
|---|
- Occurs in eukaryotes that form a glycoprotein by the transfer of a
glucosyl-mannosyl-glucosamine polysaccharide to the side-chain of an
L-asparagine residue in the sequence -Asn-Xaa-Ser- or -Asn-Xaa-Thr-
(Xaa not Pro) in nascent polypeptide chains.
- The basic oligosaccharide is the tetradecasaccharide Glc3Man9GlcNAc2.
- However, smaller oligosaccharides derived from it and
oligosaccharides with additional monosaccharide units attached may be
involved.
- Man3GlcNAc2 seems to be common for all of the oligosaccharides
involved with the terminal N-acetylglucosamine linked to the protein
L-asparagine.
- Occurs on the cytosolic face of the endoplasmic reticulum.
- The dolichol involved normally has 14-21 isoprenoid units with two
trans double-bonds at the omega end, and the rest of the double-bonds
in cis form.
- Formerly EC 2.4.1.119.
|
| Cross-references |
|---|
| BRENDA | 2.4.99.18 |
| EC2PDB | 2.4.99.18 |
| ExplorEnz | 2.4.99.18 |
| PRIAM enzyme-specific profiles | 2.4.99.18 |
| KEGG Ligand Database for Enzyme Nomenclature | 2.4.99.18 |
| IUBMB Enzyme Nomenclature | 2.4.99.18 |
| IntEnz | 2.4.99.18 |
| MEDLINE | Find literature relating to 2.4.99.18 |
| MetaCyc | 2.4.99.18 |
| Rhea expert-curated reactions | 2.4.99.18 |
| UniProtKB/Swiss-Prot |
| Q93ZY3, STT3A_ARATH; | Q2KJI2, STT3A_BOVIN; | F1PJP5, STT3A_CANLF; |
| P46977, STT3A_HUMAN; | P46978, STT3A_MOUSE; | Q6F2Z1, STT3A_ORYSJ; |
| Q5RCE2, STT3A_PONAB; | Q9FX21, STT3B_ARATH; | E2RG47, STT3B_CANLF; |
| Q8TCJ2, STT3B_HUMAN; | Q3TDQ1, STT3B_MOUSE; | Q7XQ88, STT3B_ORYSJ; |
| P46975, STT3_CAEEL; | Q54NM9, STT3_DICDI; | O94335, STT3_SCHPO; |
| P39007, STT3_YEAST; |
|
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