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ENZYME entry: EC 2.7.1.160

Accepted Name
2'-phosphotransferase.
Alternative Name(s)
Tpt1p.
yeast 2'-phosphotransferase.
Reaction catalysed
2'-phospho-[ligated tRNA] + NAD(+) <=> ADP-beta-D-ribose 1'',2''-cyclic phosphate + mature tRNA + nicotinamide
Comment(s)
  • Catalyzes the final step of tRNA splicing in the yeast Saccharomyces cerevisiae.
  • The reaction takes place in two steps: in the first step, the 2'-phosphate on the RNA substrate is ADP-ribosylated, causing the relase of nicotinamide and the formation of the reaction intermediate, ADP-ribosylated tRNA.
  • In the second step, dephosphorylated (mature) tRNA is formed along with ADP ribose 1'',2''-cyclic phosphate.
  • Highly specific for oligonucleotide substrates bearing an internal 2'-phosphate.
  • Oligonucleotides with only a terminal 5'- or 3'-phosphate are not substrates.
Cross-references
BRENDA2.7.1.160
EC2PDB2.7.1.160
ExplorEnz2.7.1.160
PRIAM enzyme-specific profiles2.7.1.160
KEGG Ligand Database for Enzyme Nomenclature2.7.1.160
IUBMB Enzyme Nomenclature2.7.1.160
IntEnz2.7.1.160
MEDLINEFind literature relating to 2.7.1.160
MetaCyc2.7.1.160
Rhea expert-curated reactions2.7.1.160
UniProtKB/Swiss-Prot
O14045, TPT1_SCHPO;  Q12272, TPT1_YEAST;  Q3ZBM7, TRPT1_BOVIN;  
Q5EAR5, TRPT1_DANRE;  Q86TN4, TRPT1_HUMAN;  Q8K3A2, TRPT1_MOUSE;  

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