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ENZYME entry: EC 2.7.1.190

Accepted Name
Aminoglycoside 2''-phosphotransferase.
Alternative Name(s)
Aminoglycoside (2'') kinase.
APH(2'').
Gentamicin kinase.
Gentamicin phosphotransferase.
Reaction catalysed
GTP + gentamicin <=> GDP + gentamicin 2''-phosphate
Cofactor(s)
Mg(2+).
Comment(s)
  • This bacterial enzyme phosphorylates many 4,6-disubstituted aminoglycoside antibiotics that have a hydroxyl group at position 2'', including kanamycin A, kanamycin B, tobramycin, dibekacin, arbekacin, amikacin, gentamicin C, sisomicin and netilmicin.
  • In most, but not all, cases the phosphorylation confers resistance against the antibiotic.
  • Some forms of the enzyme use ATP as a phosphate donor in appreciable amount.
  • The enzyme is often found as a bifunctional enzyme that also catalyzes 6'-aminoglycoside N-acetyltransferase activity.
  • The bifunctional enzyme is the most clinically important aminoglycoside-modifying enzyme in Gram-positive bacteria, responsible for high-level resistance in both Enterococci and Staphylococci.
Cross-references
BRENDA2.7.1.190
EC2PDB2.7.1.190
ExplorEnz2.7.1.190
PRIAM enzyme-specific profiles2.7.1.190
KEGG Ligand Database for Enzyme Nomenclature2.7.1.190
IUBMB Enzyme Nomenclature2.7.1.190
IntEnz2.7.1.190
MEDLINEFind literature relating to 2.7.1.190
MetaCyc2.7.1.190
Rhea expert-curated reactions2.7.1.190
UniProtKB/Swiss-Prot
P0A0C2, AACA_ENTFA;  Q52S23, AACA_ENTFC;  P0A0C0, AACA_STAAM;  
P0A0C1, AACA_STAAU;  Q5HMP3, AACA_STAEQ;  Q4L605, AACA_STAHJ;  
Q7ATH9, AACA_STASC;  Q7ATH7, AACA_STAWA;  

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