ENZYME entry: EC 3.4.24.3
Accepted Name |
microbial collagenase.
|
Alternative Name(s) |
clostridiopeptidase A. |
Clostridium histolyticum collagenase. |
collagenase A. |
collagenase I. |
Reaction catalysed |
Digestion of native collagen in the triple helical region at Xaa-|-Gly bonds. With synthetic peptides, a preference is shown for Gly at P3 and P1', Pro and Ala at P2 and P2', and hydroxyproline, Ala or Arg at P3' |
Comment(s) |
- Six species of metalloendopeptidase acting on native collagen can be
isolated from the medium of Clostridium histolyticum.
- Class I has forms alpha (68 kDa), beta (115 kDa) and gamma (79 kDa);
class II has delta (100 kDa), epsilon (110 kDa) and zeta (125 kDa).
- The two classes are immunologically crossreactive, but have
significantly different sequences, and different specificities such
that their actions on collagen are complementary.
- The enzymes also act as peptidyl-tripeptidases.
- Variants of the enzyme have been purified from Bacillus cereus,
Empedobacter collagenolyticum, Pseudomonas marinoglutinosa,
and species of Vibrio, Vibrio B-30 (ATCC 21250) and V.alginolyticus
(previously Achromobacter iophagus).
- Also known from Streptomyces sp.
- Belongs to peptidase family M9.
- Formerly EC 3.4.4.19, EC 3.4.24.8 and EC 3.4.99.5.
|
Cross-references |
BRENDA | 3.4.24.3 |
EC2PDB | 3.4.24.3 |
ExplorEnz | 3.4.24.3 |
PRIAM enzyme-specific profiles | 3.4.24.3 |
KEGG Ligand Database for Enzyme Nomenclature | 3.4.24.3 |
IUBMB Enzyme Nomenclature | 3.4.24.3 |
IntEnz | 3.4.24.3 |
MEDLINE | Find literature relating to 3.4.24.3 |
MetaCyc | 3.4.24.3 |
Rhea expert-curated reactions | 3.4.24.3 |
UniProtKB/Swiss-Prot |
Q81BJ6, COLA_BACCR; | P43153, COLA_CLOPE; | P43154, COLA_VIBAL; |
Q9KRJ0, COLA_VIBCH; | Q56696, COLA_VIBPA; | Q8D4Y9, COLA_VIBVU; |
Q9X721, COLG_HATHI; | Q46085, COLH_HATHI; | B9J3S4, COLQ1_BACCQ; |
Q899Y1, COLT_CLOTE; |
|
View entry in original ENZYME format
View entry in raw text format (no links)
All
ENZYME /
UniProtKB/Swiss-Prot entries corresponding to 3.4.24.-
All ENZYME / UniProtKB/Swiss-Prot entries corresponding to 3.4.-.-
All ENZYME / UniProtKB/Swiss-Prot entries corresponding to 3.-.-.-