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ENZYME entry: EC 4.2.2.27

Accepted Name
Pectin monosaccharide-lyase.
Alternative Name(s)
Exo-pectin lyase.
Reaction catalysed
(1,4-alpha-D-galacturonosyl methyl ester)(n) <=> (1,4-alpha-D-galacturonosyl methyl ester)(n-1) + 4-deoxy-6-O-methyl-L-threo-hex-4-enopyranuronate
Comment(s)
  • The enzyme, isolated from the fungus Aspergillus giganteus, acts on the non-reducing end of methyl-esterified polygalacturonan, releasing either 4-deoxy(-)-L-threo-hex-4-enopyranuronate or 4-deoxy-6- O-methyl-L-threo-hex-4-enopyranuronate.
  • The enzyme is stimulated by divalent cations, with Co(2+) having the strongest effect.
  • It is able to act on substrates as short as a disaccharide, and was active on substrates with degrees of methyl esterification ranging between 34% and 90%.
Cross-references
BRENDA4.2.2.27
EC2PDB4.2.2.27
ExplorEnz4.2.2.27
PRIAM enzyme-specific profiles4.2.2.27
KEGG Ligand Database for Enzyme Nomenclature4.2.2.27
IUBMB Enzyme Nomenclature4.2.2.27
IntEnz4.2.2.27
MEDLINEFind literature relating to 4.2.2.27
MetaCyc4.2.2.27
Rhea expert-curated reactions4.2.2.27

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All ENZYME / UniProtKB/Swiss-Prot entries corresponding to 4.2.2.-
All ENZYME / UniProtKB/Swiss-Prot entries corresponding to 4.2.-.-
All ENZYME / UniProtKB/Swiss-Prot entries corresponding to 4.-.-.-