| Accepted Name |
|---|
| ribonuclease U2
|
| Alternative Name(s) |
|---|
| RNase U2 |
| Reaction catalysed |
|---|
- [RNA] containing adenosine + H2O = an [RNA fragment]-3'-adenosine-3'-phosphate + a 5'-hydroxy-ribonucleotide-3'-[RNA fragment]
- [RNA] containing guanosine + H2O = an [RNA fragment]-3'-guanosine-3'-phosphate + a 5'-hydroxy-ribonucleotide-3'-[RNA fragment]
|
| Comment(s) |
|---|
- The enzyme secreted by the fungus Ustilago sphaerogena cleaves at the
3'-phosphate group of purines, and catalyzes a two-stage
endonucleolytic cleavage.
- The first reaction produces 5'-hydroxy-phosphooligonucletides and
3'-phosphooligonucleotides ending in Ap or Gp with 2',3'-cyclic
phosphodiester, which are released from the enzyme.
- The enzyme then hydrolyzes these cyclic compounds in a second
reaction that takes place only when all the susceptible
3',5'-phosphodiester bonds have been cyclised.
- The second reaction is a reversal of the first reaction using the
hydroxyl group of water instead of the 5'-hydroxyl group of ribose.
- The overall process is that of a phosphorus-oxygen lyase followed by
hydrolysis to form the 3'-nucleotides.
- Formerly EC 3.1.27.4.
|
| Cross-references |
|---|
| BRENDA | 4.6.1.20 |
| EC2PDB | 4.6.1.20 |
| ExplorEnz | 4.6.1.20 |
| PRIAM enzyme-specific profiles | 4.6.1.20 |
| KEGG Ligand Database for Enzyme Nomenclature | 4.6.1.20 |
| IUBMB Enzyme Nomenclature | 4.6.1.20 |
| IntEnz | 4.6.1.20 |
| MEDLINE | Find literature relating to 4.6.1.20 |
| MetaCyc | 4.6.1.20 |
| Rhea expert-curated reactions | 4.6.1.20 |
| UniProtKB/Swiss-Prot |
|
View entry in original ENZYME format
View entry in raw text format (no links)
All
ENZYME /
UniProtKB/Swiss-Prot entries corresponding to 4.6.1.-
All ENZYME / UniProtKB/Swiss-Prot entries corresponding to 4.6.-.-
All ENZYME / UniProtKB/Swiss-Prot entries corresponding to 4.-.-.-
