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ENZYME entry: EC

Accepted Name
Enterobacter ribonuclease
Reaction catalysed
RNA containing adenosine-cytidine + H2O <=> an [RNA fragment]-3'-cytidine-3'-phosphate + a 5'-a hydroxy-adenosine -3'-[RNA fragment]
  • Preference for cleavage at Cp-A bonds.
  • Homopolymers of A, U or G are not hydrolyzed.
  • CpG bonds are hydrolyzed less well and there is no detectable hydrolysis between two purines or two pyrimidines.
  • The enzyme catalyzes a two-stage endonucleolytic cleavage.
  • The first reaction produces 5'-hydroxy-phosphooligonucletides and 3'-phosphooligonucleotides ending a with 2',3'-cyclic phosphodiester, which are released from the enzyme.
  • The enzyme then hydrolyzes these cyclic compounds in a second reaction that takes place only when all the susceptible 3',5'-phosphodiester bonds have been cyclised.
  • The second reaction is a reversal of the first reaction using the hydroxyl group of water instead of the 5'-hydroxyl group of ribose.
  • The overall process is that of a phosphorus-oxygen lyase followed by hydrolysis to form the 3'-nucleotides.
  • Formerly EC
PRIAM enzyme-specific profiles4.6.1.21
KEGG Ligand Database for Enzyme Nomenclature4.6.1.21
IUBMB Enzyme Nomenclature4.6.1.21
MEDLINEFind literature relating to
Rhea expert-curated reactions4.6.1.21

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