ENZYME entry: EC 4.6.1.21
| Accepted Name |
|---|
| Enterobacter ribonuclease.
|
| Alternative Name(s) |
|---|
| (RNA)-adenosine-cytidine 5'-hydroxy-adenosoine ribonucleotide-3'-(RNA
fragment)-lyase (cyclicizing; (RNA fragment)-3'-cytidine-
2',3'-cyclophosphate-forming and hydrolyzing). |
| Reaction catalysed |
|---|
| RNA containing adenosine-cytidine + H(2)O <=> an (RNA fragment)-3'-cytidine-3'-phosphate + a 5'-a hydroxy-adenosine -3'-(RNA fragment) |
| Comment(s) |
|---|
- Preference for cleavage at Cp-A bonds.
- Homopolymers of A, U or G are not hydrolyzed.
- CpG bonds are hydrolyzed less well and there is no detectable
hydrolysis between two purines or two pyrimidines.
- The enzyme catalyzes a two-stage endonucleolytic cleavage.
- The first reaction produces 5'-hydroxy-phosphooligonucletides and
3'-phosphooligonucleotides ending a with 2',3'-cyclic phosphodiester,
which are released from the enzyme.
- The enzyme then hydrolyzes these cyclic compounds in a second
reaction that takes place only when all the susceptible
3',5'-phosphodiester bonds have been cyclised.
- The second reaction is a reversal of the first reaction using the
hydroxyl group of water instead of the 5'-hydroxyl group of ribose.
- The overall process is that of a phosphorus-oxygen lyase followed by
hydrolysis to form the 3'-nucleotides.
- Formerly EC 3.1.27.6.
|
| Cross-references |
|---|
| PROSITE | PDOC00459 |
| BRENDA | 4.6.1.21 |
| EC2PDB | 4.6.1.21 |
| ExplorEnz | 4.6.1.21 |
| PRIAM enzyme-specific profiles | 4.6.1.21 |
| KEGG Ligand Database for Enzyme Nomenclature | 4.6.1.21 |
| IUBMB Enzyme Nomenclature | 4.6.1.21 |
| IntEnz | 4.6.1.21 |
| MEDLINE | Find literature relating to 4.6.1.21 |
| MetaCyc | 4.6.1.21 |
| UniProtKB/Swiss-Prot |
|
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