| Accepted Name |
|---|
| bacteriophage T4 restriction endoribonuclease RegB
|
| Reaction catalysed |
|---|
| a [pre-mRNA]-containing guanosine-adenosine + H2O = a 5' hydroxy-guanosine-[pre-mRNA fragment] + a [pre-mRNA fragment]-3'-adenosine-3'-phosphate |
| Comment(s) |
|---|
- The enzyme from bacteriophage T4 cleaves early mRNAs between Ap and
Gp at one specific specific GpGpApGp site, favoring their further
transition to middle-phase mRNA.
- The activity is enhanced by Ribosomal S1 protein.
- The enzyme catalyzes a two-stage endonucleolytic cleavage.
- The first reaction produces 5'-hydroxy-phosphooligonucletides and
3'-phosphooligonucleotides ending with 2',3'-cyclic phosphodiester,
which are released from the enzyme.
- The enzyme then hydrolyzes these cyclic compounds in a second
reaction that takes place only when all the susceptible
3',5'-phosphodiester bonds have been cyclised.
- The second reaction is a reversal of the first reaction using the
hydroxyl group of water instead of the 5'-hydroxyl group of ribose.
- The overall process is that of a phosphorus-oxygen lyase followed by
hydrolysis to form the 3'-nucleotides.
|
| Cross-references |
|---|
| BRENDA | 4.6.1.25 |
| EC2PDB | 4.6.1.25 |
| ExplorEnz | 4.6.1.25 |
| KEGG Ligand Database for Enzyme Nomenclature | 4.6.1.25 |
| IUBMB Enzyme Nomenclature | 4.6.1.25 |
| MEDLINE | Find literature relating to 4.6.1.25 |
| MetaCyc | 4.6.1.25 |
| Rhea expert-curated reactions | 4.6.1.25 |
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