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ENZYME

ENZYME entry: EC 6.2.1.45

PURL: https://purl.expasy.org/enzyme/EC/6.2.1.45
Accepted Name
E1 ubiquitin-activating enzyme
Alternative Name(s)
ubiquitin-activating enzyme E1
Reaction catalysed
ATP + ubiquitin + [E1 ubiquitin-activating enzyme]-L-cysteine = AMP + diphosphate + S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine
Comment(s)
  • Catalyzes the ATP-dependent activation of ubiquitin through the formation of a thioester bond between the C-terminal glycine of ubiquitin and the sulfhydryl side group of a cysteine residue in the E1 protein.
  • The two-step reaction consists of the ATP-dependent formation of an E1-ubiquitin adenylate intermediate in which the C-terminal glycine of ubiquitin is bound to AMP via an acyl-phosphate linkage, then followed by the conversion to an E1-ubiquitin thioester bond via the cysteine residue on E1 in the second step.
  • Formerly EC 6.3.2.19 and EC 6.3.2.21.
Cross-references
BRENDA6.2.1.45
EC2PDB6.2.1.45
ExplorEnz6.2.1.45
KEGG Ligand Database for Enzyme Nomenclature6.2.1.45
IUBMB Enzyme Nomenclature6.2.1.45
MEDLINEFind literature relating to 6.2.1.45
MetaCyc6.2.1.45
Rhea expert-curated reactions6.2.1.45
UniProtKB/Swiss-Prot
P31254, UBA1Y_MOUSEA3KMV5, UBA1_BOVINP52495, UBA1_CANAW
Q55C16, UBA1_DICDIP22314, UBA1_HUMANQ02053, UBA1_MOUSE
Q29504, UBA1_RABITQ5U300, UBA1_RATO94609, UBA1_SCHPO
P22515, UBA1_YEASTA0AVT1, UBA6_HUMANQ8C7R4, UBA6_MOUSE
P93028, UBE11_ARATHP20973, UBE11_WHEATP92974, UBE12_ARATH
P31251, UBE12_WHEATP31252, UBE13_WHEATP31255, UBE1Y_OSPRU

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All ENZYME / UniProtKB/Swiss-Prot entries corresponding to 6.2.1.-
All ENZYME / UniProtKB/Swiss-Prot entries corresponding to 6.2.-.-
All ENZYME / UniProtKB/Swiss-Prot entries corresponding to 6.-.-.-