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ENZYME entry: EC 6.5.1.3

Accepted Name
RNA ligase (ATP).
Alternative Name(s)
Polyribonucleotide synthase (ATP).
Ribonucleic ligase.
Reaction catalysed
ATP + (ribonucleotide)(n)-3'-hydroxyl + 5'-phospho-(ribonucleotide)(m) <=> (ribonucleotide)(n+m) + AMP + diphosphate
Comment(s)
  • The enzyme catalyzes the ligation of RNA strands with 3'-hydroxyl and 5'-phosphate termini, forming a phosphodiester and sealing certain types of single-strand breaks in RNA.
  • Catalysis occurs by a three-step mechanism, starting with the activation of the enzyme by ATP, forming a phosphoramide bond between adenylate and a lysine residue.
  • The adenylate group is then transferred to the 5'-phosphate terminus of the substrate, forming the capped structure 5'-(5'-diphosphoadenosine)-(RNA).
  • Finally, the enzyme catalyzes a nucleophilic attack of the 3'-OH terminus on the capped terminus, which results in formation of the phosphodiester bond and release of the adenylate.
Cross-references
BRENDA6.5.1.3
EC2PDB6.5.1.3
ExplorEnz6.5.1.3
PRIAM enzyme-specific profiles6.5.1.3
KEGG Ligand Database for Enzyme Nomenclature6.5.1.3
IUBMB Enzyme Nomenclature6.5.1.3
IntEnz6.5.1.3
MEDLINEFind literature relating to 6.5.1.3
MetaCyc6.5.1.3
Rhea expert-curated reactions6.5.1.3
UniProtKB/Swiss-Prot
P86927, RLGM1_TRYB2;  P86926, RLGM1_TRYBB;  P86925, RLGM2_TRYB2;  
P86924, RLGM2_TRYBB;  P32277, RLIG2_BPT4;  P00971, RLIG_BPT4;  
P41476, RNL1_NPVAC;  Q0WL81, RNL_ARATH;  P43075, TRNL_CANAL;  
Q10313, TRNL_SCHPO;  P09880, TRNL_YEAST;  

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