- The enzyme catalyzes the ligation of RNA strands with 3'-hydroxyl and
5'-phosphate termini, forming a phosphodiester and sealing certain
types of single-strand breaks in RNA.
- Catalysis occurs by a three-step mechanism, starting with the
activation of the enzyme by ATP, forming a phosphoramide bond between
adenylate and a lysine residue.
- The adenylate group is then transferred to the 5'-phosphate terminus
of the substrate, forming the capped structure
5'-(5'-diphosphoadenosine)-(RNA).
- Finally, the enzyme catalyzes a nucleophilic attack of the 3'-OH
terminus on the capped terminus, which results in formation of the
phosphodiester bond and release of the adenylate.
|
| UniProtKB/Swiss-Prot |
| P86927, RLGM1_TRYB2; | P86926, RLGM1_TRYBB; | P86925, RLGM2_TRYB2; |
| P86924, RLGM2_TRYBB; | P32277, RLIG2_BPT4; | P00971, RLIG_BPT4; |
| P41476, RNL1_NPVAC; | Q0WL81, RNL_ARATH; | B0WCT9, RTCB1_CULQU; |
| B0EAV2, RTCB1_ENTDS; | C4M244, RTCB1_ENTHI; | B0XKF3, RTCB2_CULQU; |
| B0EIW5, RTCB2_ENTDS; | C4M6T2, RTCB2_ENTHI; | Q17FP1, RTCB_AEDAE; |
| Q7Q412, RTCB_ANOGA; | Q5E9T9, RTCB_BOVIN; | C3YN79, RTCB_BRAFL; |
| A8QC60, RTCB_BRUMA; | P90838, RTCB_CAEEL; | A8JC00, RTCB_CHLRE; |
| Q6NZS4, RTCB_DANRE; | Q54Y09, RTCB_DICDI; | Q9VIW7, RTCB_DROME; |
| Q9Y3I0, RTCB_HUMAN; | Q4R6X4, RTCB_MACFA; | C1E9Y5, RTCB_MICCC; |
| C1MI97, RTCB_MICPC; | A9UXG6, RTCB_MONBE; | Q99LF4, RTCB_MOUSE; |
| A7RKF6, RTCB_NEMVE; | A4S3S3, RTCB_OSTLU; | Q00ZY2, RTCB_OSTTA; |
| A9CB42, RTCB_PAPAN; | Q19PY3, RTCB_PIG; | Q4YUZ9, RTCB_PLABA; |
| Q8IIU6, RTCB_PLAF7; | B3L4K9, RTCB_PLAKH; | Q7RI54, RTCB_PLAYO; |
| Q6AYT3, RTCB_RAT; | B8LBM8, RTCB_THAPS; | Q4U923, RTCB_THEAN; |
| Q4N1R8, RTCB_THEPA; | B3RID0, RTCB_TRIAD; | Q561P3, RTCB_XENTR; |
| P43075, TRNL_CANAL; | Q10313, TRNL_SCHPO; | P09880, TRNL_YEAST; |
|