ENZYME entry: EC 6.5.1.3
Accepted Name |
RNA ligase (ATP).
|
Alternative Name(s) |
Polyribonucleotide synthase (ATP). |
Ribonucleic ligase. |
Reaction catalysed |
ATP + (ribonucleotide)(n)-3'-hydroxyl + 5'-phospho-(ribonucleotide)(m) <=> (ribonucleotide)(n+m) + AMP + diphosphate |
Comment(s) |
- The enzyme catalyzes the ligation of RNA strands with 3'-hydroxyl and
5'-phosphate termini, forming a phosphodiester and sealing certain
types of single-strand breaks in RNA.
- Catalysis occurs by a three-step mechanism, starting with the
activation of the enzyme by ATP, forming a phosphoramide bond between
adenylate and a lysine residue.
- The adenylate group is then transferred to the 5'-phosphate terminus
of the substrate, forming the capped structure
5'-(5'-diphosphoadenosine)-(RNA).
- Finally, the enzyme catalyzes a nucleophilic attack of the 3'-OH
terminus on the capped terminus, which results in formation of the
phosphodiester bond and release of the adenylate.
|
Cross-references |
BRENDA | 6.5.1.3 |
EC2PDB | 6.5.1.3 |
ExplorEnz | 6.5.1.3 |
PRIAM enzyme-specific profiles | 6.5.1.3 |
KEGG Ligand Database for Enzyme Nomenclature | 6.5.1.3 |
IUBMB Enzyme Nomenclature | 6.5.1.3 |
IntEnz | 6.5.1.3 |
MEDLINE | Find literature relating to 6.5.1.3 |
MetaCyc | 6.5.1.3 |
UniProtKB/Swiss-Prot |
P86927, RLGM1_TRYB2; | P86926, RLGM1_TRYBB; | P86925, RLGM2_TRYB2; |
P86924, RLGM2_TRYBB; | P32277, RLIG2_BPT4; | P00971, RLIG_BPT4; |
P41476, RNL1_NPVAC; | Q0WL81, RNL_ARATH; | P43075, TRNL_CANAL; |
Q10313, TRNL_SCHPO; | P09880, TRNL_YEAST; |
|
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