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ENZYME

ENZYME entry: EC 6.5.1.3

PURL: https://purl.expasy.org/enzyme/EC/6.5.1.3
Accepted Name
RNA ligase (ATP)
Alternative Name(s)
polyribonucleotide synthase (ATP)
ribonucleic ligase
Reaction catalysed
ATP + (ribonucleotide)n-3'-hydroxyl + 5'-phospho-(ribonucleotide)m = (ribonucleotide)n+m + AMP + diphosphate
Comment(s)
  • The enzyme catalyzes the ligation of RNA strands with 3'-hydroxyl and 5'-phosphate termini, forming a phosphodiester and sealing certain types of single-strand breaks in RNA.
  • Catalysis occurs by a three-step mechanism, starting with the activation of the enzyme by ATP, forming a phosphoramide bond between adenylate and a lysine residue.
  • The adenylate group is then transferred to the 5'-phosphate terminus of the substrate, forming the capped structure 5'-(5'-diphosphoadenosine)-[RNA].
  • Finally, the enzyme catalyzes a nucleophilic attack of the 3'-OH terminus on the capped terminus, which results in formation of the phosphodiester bond and release of the adenylate.
Cross-references
BRENDA6.5.1.3
EC2PDB6.5.1.3
ExplorEnz6.5.1.3
KEGG Ligand Database for Enzyme Nomenclature6.5.1.3
IUBMB Enzyme Nomenclature6.5.1.3
MEDLINEFind literature relating to 6.5.1.3
MetaCyc6.5.1.3
Rhea expert-curated reactions6.5.1.3
UniProtKB/Swiss-Prot
P86927, RLGM1_TRYB2P86926, RLGM1_TRYBBP86925, RLGM2_TRYB2
P86924, RLGM2_TRYBBQ2KJ69, RLIG1_BOVINQ5PR55, RLIG1_DANRE
Q8N999, RLIG1_HUMANQ8BHN7, RLIG1_MOUSEQ5RFG5, RLIG1_PONAB
Q6AXM9, RLIG1_RATP32277, RLIG2_BPT4P00971, RLIG_BPT4
C0HM52, RLIG_BPTS2P41476, RNL1_NPVACQ0WL81, RNL_ARATH
P43075, TRNL_CANALQ10313, TRNL_SCHPOP09880, TRNL_YEAST

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