ENZYME entry: EC

Accepted Name
Multisite-specific tRNA:(cytosine-C(5))-methyltransferase.
Reaction catalysed
  • S-adenosyl-L-methionine + cytosine(34) in tRNA precursor <=> S-adenosyl-L-homocysteine + 5-methylcytosine(34) in tRNA precursor
  • S-adenosyl-L-methionine + cytosine(40) in tRNA precursor <=> S-adenosyl-L-homocysteine + 5-methylcytosine(40) in tRNA precursor
  • S-adenosyl-L-methionine + cytosine(48) in tRNA <=> S-adenosyl-L-homocysteine + 5-methylcytosine(48) in tRNA
  • S-adenosyl-L-methionine + cytosine(49) in tRNA <=> S-adenosyl-L-homocysteine + 5-methylcytosine(49) in tRNA
  • The enzyme from Saccharomyces cerevisiae is responsible for complete 5-methylcytosine methylations of yeast tRNA.
  • The incidence of modification depends on the cytosine position in tRNA.
  • At positions 34 and 40, 5-methylcytosine is found only in two yeast tRNAs (tRNA(Leu)(CUA) and tRNA(Phe)(GAA), respectively), whereas most other elongator yeast tRNAs bear either 5-methylcytosine(48) or 5-methylcytosine(49), but never both in the same tRNA molecule.
  • The formation of 5-methylcytosine(34) and 5-methylcytosine(40) is a strictly intron-dependent process, whereas the formation of 5-methylcytosine(48) and 5-methylcytosine(49) is an intron- independent process.
  • Formerly EC
PRIAM enzyme-specific profiles2.1.1.202
KEGG Ligand Database for Enzyme Nomenclature2.1.1.202
IUBMB Enzyme Nomenclature2.1.1.202
MEDLINEFind literature relating to
P38205, NCL1_YEAST

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