ENZYME entry: EC 2.1.1.202

Accepted Name
Multisite-specific tRNA:(cytosine-C(5))-methyltransferase.
Reaction catalysed
  • S-adenosyl-L-methionine + cytosine(34) in tRNA precursor <=> S-adenosyl-L-homocysteine + 5-methylcytosine(34) in tRNA precursor
  • S-adenosyl-L-methionine + cytosine(40) in tRNA precursor <=> S-adenosyl-L-homocysteine + 5-methylcytosine(40) in tRNA precursor
  • S-adenosyl-L-methionine + cytosine(48) in tRNA <=> S-adenosyl-L-homocysteine + 5-methylcytosine(48) in tRNA
  • S-adenosyl-L-methionine + cytosine(49) in tRNA <=> S-adenosyl-L-homocysteine + 5-methylcytosine(49) in tRNA
Comment(s)
  • The enzyme from Saccharomyces cerevisiae is responsible for complete 5-methylcytosine methylations of yeast tRNA.
  • The incidence of modification depends on the cytosine position in tRNA.
  • At positions 34 and 40, 5-methylcytosine is found only in two yeast tRNAs (tRNA(Leu)(CUA) and tRNA(Phe)(GAA), respectively), whereas most other elongator yeast tRNAs bear either 5-methylcytosine(48) or 5-methylcytosine(49), but never both in the same tRNA molecule.
  • The formation of 5-methylcytosine(34) and 5-methylcytosine(40) is a strictly intron-dependent process, whereas the formation of 5-methylcytosine(48) and 5-methylcytosine(49) is an intron- independent process.
  • Formerly EC 2.1.1.29.
Cross-references
BRENDA2.1.1.202
EC2PDB2.1.1.202
ExplorEnz2.1.1.202
PRIAM enzyme-specific profiles2.1.1.202
KEGG Ligand Database for Enzyme Nomenclature2.1.1.202
IUBMB Enzyme Nomenclature2.1.1.202
IntEnz2.1.1.202
MEDLINEFind literature relating to 2.1.1.202
MetaCyc2.1.1.202
UniProtKB/Swiss-Prot
P38205, NCL1_YEAST

View entry in original ENZYME format
View entry in raw text format (no links)

All UniProtKB/Swiss-Prot entries referenced in this entry, with possibility to download in different formats, align etc.
All ENZYME / UniProtKB/Swiss-Prot entries corresponding to 2.1.1.-
All ENZYME / UniProtKB/Swiss-Prot entries corresponding to 2.1.-.-
All ENZYME / UniProtKB/Swiss-Prot entries corresponding to 2.-.-.-