ENZYME

ENZYME entry: EC 2.8.2.30

Accepted Name

[Heparan sulfate]-glucosamine 3-sulfotransferase 3.

Alternative Name(s)

3-OST-3.

Glucosaminyl 3-O-sulfotransferase 3.

Heparan sulfate D-glucosaminyl 3-O-sulfotransferase 3.

Reaction catalysed

3'-phosphoadenylyl sulfate + [heparan sulfate]-glucosamine <=> adenosine 3',5'-bisphosphate + [heparan sulfate]-glucosamine 3-sulfate

Comment(s)

Two major substrates contain the tetrasaccharides: -> undetermined 2-sulfo-uronic acid->GlcN2S->IdoA2S->GlcN*-> and -> undetermined 2-sulfo-uronic acid->GlcN2S->IdoA2S->GlcN6S*-> with modification of the N-unsubstituted glucosamine residue (shown with an asterisk).
Modification of selected sequences containing N-sulfo-glucosamine residues cannot yet be excluded.
The 3-O-sulfated heparan sulfate can be utilized by Herpes simplex virus type 1 as an entry receptor to infect the target cells.
There are two isozymes, known as 3-OST-3(A) and 3-OST-3(B), which have identical catalytic domains but are encoded by different mammalian genes.
The specificity of this enzyme differs from that of the other [heparan sulfate]-glucosamine 3-sulfotransferases.
It is inefficient at modifying precursors of the antithrombin binding site (in contrast to EC 2.8.2.23) and it does not modify glucosamine preceded by GlcA2S (unlike EC 2.8.2.29).

Cross-references

BRENDA

EC2PDB

ExplorEnz

PRIAM enzyme-specific profiles

KEGG Ligand Database for Enzyme Nomenclature

IUBMB Enzyme Nomenclature