ENZYME

ENZYME entry: EC 2.8.2.23

Accepted Name

[Heparan sulfate]-glucosamine 3-sulfotransferase 1.

Alternative Name(s)

3'-phosphoadenylyl-sulfate:heparin-glucosamine 3-O-sulfotransferase.

3-OST-1.

Glucosaminyl 3-O-sulfotransferase.

Heparan sulfate D-glucosaminyl 3-O-sulfotransferase.

Heparin-glucosamine 3-O-sulfotransferase.

Reaction catalysed

3'-phosphoadenylyl sulfate + [heparan sulfate]-glucosamine <=> adenosine 3',5'-bisphosphate + [heparan sulfate]-glucosamine 3-sulfate

Comment(s)

This enzyme differs from EC 2.8.2.29 and EC 2.8.2.30 by being the most selective for a precursor of the antithrombin-binding site.
It has a minimal acceptor sequence of: -> GlcNAc6S->GlcA-> GlcN2S*+-6S->IdoA2S->GlcN2S->.
It can also modify other precursor sequences within heparan sulfate but this action does not create functional antithrombin-binding sites.
These precursors are variants of the consensus sequence: -> Glc(N2S>NAc)+-6S->GlcA->GlcN2S*+-6S->GlcA>IdoA+-2S-> Glc(N2S/NAc)+-6S->.
If the heparan sulfate substrate lacks 2-O-sulfation of GlcA residues, then enzyme specificity is expanded to modify selected glucosamine residues preceded by IdoA as well as GlcA.

Cross-references

BRENDA

EC2PDB

ExplorEnz

PRIAM enzyme-specific profiles

KEGG Ligand Database for Enzyme Nomenclature

IUBMB Enzyme Nomenclature

IntEnz

MEDLINE

MetaCyc

UniProtKB/Swiss-Prot

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