ENZYME entry: EC 6.5.1.1

Accepted Name
DNA ligase (ATP).
Alternative Name(s)
DNA joinase.
DNA repair enzyme.
Polydeoxyribonucleotide synthase (ATP).
Polynucleotide ligase (ATP).
Sealase.
Reaction catalysed
ATP + (deoxyribonucleotide)(n)-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)(m) <=> (deoxyribonucleotide)(n+m) + AMP + diphosphate
Comment(s)
  • The enzyme catalyzes the ligation of DNA strands with 3'-hydroxyl and 5'-phosphate termini, forming a phosphodiester and sealing certain types of single-strand breaks in duplex DNA.
  • Catalysis occurs by a three-step mechanism, starting with the activation of the enzyme by ATP, forming a phosphoramide bond between adenylate and a lysine residue.
  • The adenylate group is then transferred to the 5'-phosphate terminus of the substrate, forming the capped structure 5'-(5'-diphosphoadenosine)-(DNA).
  • Finally, the enzyme catalyzes a nucleophilic attack of the 3'-OH terminus on the capped terminus, which results in formation of the phosphodiester bond and release of the adenylate.
  • RNA can also act as substrate, to some extent.
  • Cf. EC 6.5.1.2, EC 6.5.1.6 and EC 6.5.1.7.
Cross-references
PROSITEPDOC00295
BRENDA6.5.1.1
EC2PDB6.5.1.1
ExplorEnz6.5.1.1
PRIAM enzyme-specific profiles6.5.1.1
KEGG Ligand Database for Enzyme Nomenclature6.5.1.1
IUBMB Enzyme Nomenclature6.5.1.1
IntEnz6.5.1.1
MEDLINEFind literature relating to 6.5.1.1
MetaCyc6.5.1.1
UniProtKB/Swiss-Prot
Q42572, DNLI1_ARATH;  Q27474, DNLI1_CAEEL;  Q869E1, DNLI1_DICDI;  
Q9W1H4, DNLI1_DROME;  Q18HT7, DNLI1_HALWD;  P18858, DNLI1_HUMAN;  
B1L3K8, DNLI1_KORCO;  Q8TSR7, DNLI1_METAC;  Q46C04, DNLI1_METBF;  
Q8PVR7, DNLI1_METMA;  P37913, DNLI1_MOUSE;  Q9JHY8, DNLI1_RAT;  
P12000, DNLI1_SCHPO;  A1RXA6, DNLI1_THEPD;  P51892, DNLI1_XENLA;  
P04819, DNLI1_YEAST;  Q18GX5, DNLI2_HALWD;  B1L3V2, DNLI2_KORCO;  
Q8TMT1, DNLI2_METAC;  Q46BA3, DNLI2_METBF;  Q8PTK1, DNLI2_METMA;  
A1RY72, DNLI2_THEPD;  Q54QM4, DNLI3_DICDI;  P49916, DNLI3_HUMAN;  
P97386, DNLI3_MOUSE;  Q9C1W9, DNLI3_SCHPO;  Q9LL84, DNLI4_ARATH;  
Q75CA4, DNLI4_ASHGO;  Q4WVG8, DNLI4_ASPFU;  Q2U6A1, DNLI4_ASPOR;  
P52496, DNLI4_CANAL;  Q6FVD8, DNLI4_CANGA;  Q90YB1, DNLI4_CHICK;  
Q1DKE7, DNLI4_COCIM;  A8N936, DNLI4_COPC7;  Q7Z7W5, DNLI4_COPCI;  
P0CN09, DNLI4_CRYNB;  P0CN08, DNLI4_CRYNJ;  Q54CR9, DNLI4_DICDI;  
Q5BH83, DNLI4_EMENI;  P49917, DNLI4_HUMAN;  Q6CSH0, DNLI4_KLULA;  
Q8BTF7, DNLI4_MOUSE;  Q7SB49, DNLI4_NEUCR;  Q7X7E9, DNLI4_ORYSJ;  
Q0UCI9, DNLI4_PHANO;  Q5R6L3, DNLI4_PONAB;  O74833, DNLI4_SCHPO;  
Q6C8A3, DNLI4_YARLI;  Q08387, DNLI4_YEAST;  Q02093, DNLI_ACIAM;  
Q9YD18, DNLI_AERPE;  B8JBM1, DNLI_ANAD2;  Q2IH69, DNLI_ANADE;  
A7HID3, DNLI_ANADF;  B4UIS1, DNLI_ANASK;  O67398, DNLI_AQUAE;  
O29632, DNLI_ARCFU;  P35970, DNLI_ASFB7;  P0C992, DNLI_ASFK5;  
P26813, DNLI_ASFM2;  P0C990, DNLI_ASFP4;  P0C991, DNLI_ASFWA;  
C5BW79, DNLI_BEUC1;  P07717, DNLI_BPT3;  P00970, DNLI_BPT4;  
P19088, DNLI_BPT6;  P00969, DNLI_BPT7;  A8MDU6, DNLI_CALMQ;  
A0RWD6, DNLI_CENSY;  B5YD88, DNLI_DICT6;  B8DZX8, DNLI_DICTD;  
Q67480, DNLI_FOWPN;  P44121, DNLI_HAEIN;  B9LUZ9, DNLI_HALLT;  
Q5V4R0, DNLI_HALMA;  B0R4C9, DNLI_HALS3;  Q9HR35, DNLI_HALSA;  
D4GYZ4, DNLI_HALVD;  A8AB20, DNLI_IGNH4;  A6WG40, DNLI_KINRD;  
Q1II25, DNLI_KORVE;  A6UVC4, DNLI_META3;  Q0W0X1, DNLI_METAR;  
A7IAA9, DNLI_METB6;  Q12X08, DNLI_METBU;  Q2FTH4, DNLI_METHJ;  
Q57635, DNLI_METJA;  Q8TWN3, DNLI_METKA;  A2SR38, DNLI_METLZ;  
A4FY19, DNLI_METM5;  A9AAX4, DNLI_METM6;  A6VFQ9, DNLI_METM7;  
A3CWP1, DNLI_METMJ;  Q6LYM1, DNLI_METMP;  B8GGB8, DNLI_METPE;  
A5UKX2, DNLI_METS3;  A4YD25, DNLI_METS5;  Q2NHF9, DNLI_METST;  
P54875, DNLI_METTF;  Q50566, DNLI_METTH;  A0B7F9, DNLI_METTP;  
A6UNY0, DNLI_METVS;  A0QJL0, DNLI_MYCA1;  Q7TTR7, DNLI_MYCBO;  
A1KN60, DNLI_MYCBP;  C1AGI1, DNLI_MYCBT;  A4TDS2, DNLI_MYCGI;  
B2HHE1, DNLI_MYCMM;  Q73V97, DNLI_MYCPA;  A0QUP1, DNLI_MYCS2;  
A3PXE2, DNLI_MYCSJ;  A1UDY9, DNLI_MYCSK;  Q1BB26, DNLI_MYCSS;  
A5U776, DNLI_MYCTA;  P9WNV4, DNLI_MYCTO;  P9WNV5, DNLI_MYCTU;  
A0PTC0, DNLI_MYCUA;  A1T6P2, DNLI_MYCVP;  Q74M64, DNLI_NANEQ;  
Q3IPX0, DNLI_NATPD;  A9A3K1, DNLI_NITMS;  Q5YNY7, DNLI_NOCFA;  
A1SKL2, DNLI_NOCSJ;  Q9YMV2, DNLI_NPVLD;  B1ZZL9, DNLI_OPITP;  
C0QSL7, DNLI_PERMH;  Q6L195, DNLI_PICTO;  B8HCE1, DNLI_PSECP;  
P0CL75, DNLI_PYRAB;  O93723, DNLI_PYRAE;  A4WH24, DNLI_PYRAR;  
P0CL74, DNLI_PYRAY;  A3MS62, DNLI_PYRCJ;  P56709, DNLI_PYRFU;  
O59288, DNLI_PYRHO;  A1RTK4, DNLI_PYRIL;  B1YA52, DNLI_PYRNV;  
Q0SG75, DNLI_RHOJR;  C1AX87, DNLI_RHOOB;  A4F870, DNLI_SACEN;  
A9GU24, DNLI_SORC5;  Q826P6, DNLI_STRAW;  Q9FCB1, DNLI_STRCO;  
B1W5J2, DNLI_STRGG;  Q4JAM1, DNLI_SULAC;  C3MZR1, DNLI_SULIA;  
C4KIZ2, DNLI_SULIK;  C3MJ14, DNLI_SULIL;  C3MYD2, DNLI_SULIM;  
C3NF77, DNLI_SULIN;  C3N834, DNLI_SULIY;  Q9P9K9, DNLI_SULSH;  
Q980T8, DNLI_SULSO;  Q976G4, DNLI_SULTO;  Q9HJ26, DNLI_THEAC;  
Q979A1, DNLI_THEVO;  O57250, DNLI_VACCA;  P20492, DNLI_VACCC;  
P16272, DNLI_VACCW;  P33798, DNLI_VAR67;  F4HPZ9, LIG6_ARATH;  
A0R5T2, LIGC1_MYCS2;  A0R5T3, LIGC2_MYCS2;  L0TDE1, LIGC_MYCTU;  
O34398, LIGD_BACSU;  P59971, LIGD_MYCBO;  A0R3R7, LIGD_MYCS2;  
P9WNV2, LIGD_MYCTO;  P9WNV3, LIGD_MYCTU;  Q9I1X7, LIGD_PSEAE;  
O54003, REP75_PYRAB;  P03631, REPA_BPPHS;  P19189, REP_BPCHP;  
P69546, REP_BPF1;  P69545, REP_BPFD;  P15419, REP_BPI22;  
O80302, REP_BPIF1;  P03660, REP_BPIKE;  P69547, REP_BPM13;  
P03627, REP_BPPF3;  Q38617, REP_BPPHL;  Q9G050, REP_BPPHM;  
P11334, REP_SPV4;  

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