ENZYME entry: EC 1.1.98.6

Accepted Name

ribonucleoside-triphosphate reductase (formate)

Alternative Name(s)

anaerobic ribonucleoside-triphosphate reductase

Reaction catalysed

a ribonucleoside 5'-triphosphate + formate + H(+) = a 2'-deoxyribonucleoside 5'-triphosphate + CO2 + H2O

Comment(s)

The enzyme, which is expressed in the bacterium Escherichia coli during anaerobic growth, contains an iron sulfur center.
The active form of the enzyme contains an oxygen-sensitive glycyl (1-amino-2-oxoethan-1-yl) radical that is generated by the activating enzyme NrdG via chemistry involving S-adenosylmethionine (SAM) and a [4Fe-4S] cluster.
The glycyl radical is involved in generation of a transient thiyl (sulfanyl) radical on a cysteine residue, which attacks the substrate, forming a ribonucleotide 3'-radical, followed by water loss to form a ketyl (alpha-oxoalkyl) radical.
The ketyl radical gains an electron from a cysteine residue and a proton from formic acid, forming 3'-keto-deoxyribonucleotide and generating a thiosulfuranyl (1lambda(4)-disulfan-1-yl) radical bridge between methionine and cysteine residues.
Oxidation of formate by the thiosulfuranyl radical results in the release of CO2 and regeneration of the thiyl radical. cf. EC 1.17.4.1 and EC 1.17.4.2.

Cross-references

BRENDA

EC2PDB

ExplorEnz

KEGG Ligand Database for Enzyme Nomenclature

IUBMB Enzyme Nomenclature

MEDLINE

MetaCyc

Rhea expert-curated reactions

UniProtKB/Swiss-Prot

P07071, NRDD_BPT4	P28903, NRDD_ECOLI	P43752, NRDD_HAEIN
Q9L646, NRDD_SALTY

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