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ENZYME

ENZYME entry: EC 1.14.13.178

PURL: https://purl.expasy.org/enzyme/EC/1.14.13.178
Accepted Name
methylxanthine N(1)-demethylase
Reaction catalysed
  • caffeine + NADH + O2 + H(+) = theobromine + formaldehyde + NAD(+) + H2O
  • caffeine + NADPH + O2 + H(+) = theobromine + formaldehyde + NADP(+) + H2O
  • theophylline + NADPH + O2 + H(+) = 3-methylxanthine + formaldehyde + NADP(+) + H2O
  • theophylline + NADH + O2 + H(+) = 3-methylxanthine + formaldehyde + NAD(+) + H2O
  • 1,7-dimethylxanthine + NADH + O2 + H(+) = 7-methylxanthine + formaldehyde + NAD(+) + H2O
  • 1,7-dimethylxanthine + NADPH + O2 + H(+) = 7-methylxanthine + formaldehyde + NADP(+) + H2O
Comment(s)
  • The enzyme from the bacterium Pseudomonas putida shares an NAD(P)H-FMN reductase subunit with EC 1.14.13.179, and has a 5-fold higher activity with NADH than with NADPH.
  • Also demethylates 1-methylxantine with lower efficiency.
  • Forms part of the degradation pathway of methylxanthines.
Cross-references
BRENDA1.14.13.178
EC2PDB1.14.13.178
ExplorEnz1.14.13.178
KEGG Ligand Database for Enzyme Nomenclature1.14.13.178
IUBMB Enzyme Nomenclature1.14.13.178
MEDLINEFind literature relating to 1.14.13.178
MetaCyc1.14.13.178
Rhea expert-curated reactions1.14.13.178
UniProtKB/Swiss-Prot
H9N289, NDMA_PSEPU

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