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ENZYME entry: EC 1.14.14.139

Accepted Name
5-beta-cholestane-3-alpha,7-alpha-diol 12-alpha-hydroxylase.
Alternative Name(s)
5-beta-cholestane-3-alpha,7-alpha-diol 12-alpha-monooxygenase.
7-alpha-hydroxy-4-cholesten-3-one 12-alpha-monooxygenase.
7-alpha-hydroxycholest-4-en-3-one 12-alpha-hydroxylase.
Chenodeoxycholate 12-alpha monooxygenase.
CYP8B1.
Sterol 12-alpha-hydroxylase.
Reaction catalysed
  • 5-beta-cholestane-3-alpha,7-alpha-diol + [reduced NADPH--hemoprotein reductase] + O(2) <=> 5-beta-cholestane-3-alpha,7-alpha,12-alpha-triol + [oxidized NADPH--hemoprotein reductase] + H(2)O
  • 7-alpha-hydroxycholest-4-en-3-one + [reduced NADPH--hemoprotein reductase] + O(2) <=> 7-alpha,12-alpha-dihydroxycholest-4-en-3-one + [oxidized NADPH--hemoprotein reductase] + H(2)O
  • Chenodeoxycholate + [reduced NADPH--hemoprotein reductase] + O(2) <=> cholate + [oxidized NADPH--hemoprotein reductase] + H(2)O
Cofactor(s)
Heme-thiolate.
Comment(s)
  • A cytochrome P450 (heme-thiolate) protein found in mammals.
  • This is the key enzyme in the biosynthesis of the bile acid cholate.
  • The enzyme can also hydroxylate 5-beta-cholestane-3-alpha,7-alpha- diol at the 25 and 26 position, but to a lesser extent.
  • Formerly EC 1.14.13.95, EC 1.14.13.96 and EC 1.14.18.8.
Cross-references
PROSITEPDOC00081
BRENDA1.14.14.139
EC2PDB1.14.14.139
ExplorEnz1.14.14.139
PRIAM enzyme-specific profiles1.14.14.139
KEGG Ligand Database for Enzyme Nomenclature1.14.14.139
IUBMB Enzyme Nomenclature1.14.14.139
IntEnz1.14.14.139
MEDLINEFind literature relating to 1.14.14.139
MetaCyc1.14.14.139
Rhea expert-curated reactions1.14.14.139
UniProtKB/Swiss-Prot
Q9UNU6, CP8B1_HUMAN;  O88962, CP8B1_MOUSE;  Q7YRB2, CP8B1_PIG;  
O02766, CP8B1_RABIT;  

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