ENZYME entry: EC 1.14.14.24

Accepted Name

vitamin D 25-hydroxylase

Alternative Name(s)

CYP2R1

vitamin D2 25-hydroxylase

vitamin D3 25-hydroxylase

Reaction catalysed

calciol + O2 + reduced [NADPH--hemoprotein reductase] = calcidiol + H(+) + H2O + oxidized [NADPH--hemoprotein reductase]

Comment(s)

A microsomal enzyme isolated from human and mouse liver that bioactivates vitamin D3.
While multiple isoforms (CYP27A1, CYP2J2/3, CYP3A4, CYP2D25 and CYP2C11) are able to catalyze the reaction in vitro, only CYP2R1 is thought to catalyze the reaction in humans in vivo.
The direct electron donor to the enzyme is EC 1.6.2.4.
Formerly EC 1.14.13.159.

Cross-references

BRENDA

EC2PDB

ExplorEnz

PRIAM enzyme-specific profiles

KEGG Ligand Database for Enzyme Nomenclature

IUBMB Enzyme Nomenclature

IntEnz

MEDLINE

MetaCyc

Rhea expert-curated reactions

UniProtKB/Swiss-Prot

O46658, CP2DP_PIG

Q6VVX0, CP2R1_HUMAN

Q6VVW9, CP2R1_MOUSE

All UniProtKB/Swiss-Prot entries referenced in this entry, with possibility to download in different formats, align etc.

All ENZYME / UniProtKB/Swiss-Prot entries corresponding to 1.14.14.-
All ENZYME / UniProtKB/Swiss-Prot entries corresponding to 1.14.-.-
All ENZYME / UniProtKB/Swiss-Prot entries corresponding to 1.-.-.-